Kamel F, Krey L C
Mol Cell Endocrinol. 1982 Apr;26(1-2):151-64. doi: 10.1016/0303-7207(82)90013-2.
Enzymatically dispersed rat pituitary cells were grown in primary culture, and LHRH-stimulated LH secretion was measured. Testosterone (T) decreased and 17 beta-estradiol (E) increased pituitary responsiveness to LHRH. The effect of E on LH secretion was partly due to an increase in LH content. There was a latent period of 12 h for E and 18 h for T between the onset of steroid treatment and the manifestation of steroid action. Neither steroid was required to be continuously present in order to exert its effects. After steroid withdrawal, the effect of T persisted for 72 h and that of E for more than 96 h. The actions of both steroids were blocked by protein-synthesis inhibitors. These results are consistent with the hypothesis that steroid effects rely on a mechanism involving alterations in protein synthesis; the affected proteins may be involved in the process of LHRH action.
将大鼠垂体细胞进行酶解分散后进行原代培养，并检测促性腺激素释放激素（LHRH）刺激后的促黄体生成素（LH）分泌情况。睾酮（T）降低，而17β-雌二醇（E）增强垂体对LHRH的反应性。E对LH分泌的影响部分归因于LH含量的增加。从开始给予甾体激素治疗到甾体激素作用显现，E的潜伏期为12小时，T为18小时。两种甾体激素发挥作用均无需持续存在。撤除甾体激素后，T的作用持续72小时，E的作用持续超过96小时。两种甾体激素的作用均被蛋白质合成抑制剂阻断。这些结果与以下假说一致：甾体激素的作用依赖于一种涉及蛋白质合成改变的机制；受影响的蛋白质可能参与LHRH的作用过程。
