噬菌体T5 DNA片段的克隆。III. 在大肠杆菌微型细胞中的表达。
Cloning of bacteriophage T5 DNA fragments. III. Expression in Escherichia coli mini-cells.
作者信息
Brunel F, Davison J, Ha-Thi V, Reeve J
出版信息
Gene. 1981 Dec;16(1-3):107-18. doi: 10.1016/0378-1119(81)90067-6.
PMID:6282684
Abstract
Use has been made of the mini-cell system to study polypeptide synthesis from cloned EcoRI, HindIII and PstI fragments of T5 DNA. The correlation of certain gene products with known genes has been established, as well as the physical mapping of genes not yet identified genetically. In some cases, it has been possible to demonstrate the presence of T5 promoters on the cloned DNA fragments. The design of experiments to avoid certain artifacts inherent in the use of the mini-cell system is discussed.
摘要
利用微细胞系统研究了从T5 DNA的克隆EcoRI、HindIII和PstI片段合成多肽的过程。已确定了某些基因产物与已知基因的相关性，以及尚未进行遗传鉴定的基因的物理图谱。在某些情况下，已能够证明克隆的DNA片段上存在T5启动子。本文讨论了避免微细胞系统使用中固有的某些假象的实验设计。
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Gene. 1981 Dec;16(1-3):107-18. doi: 10.1016/0378-1119(81)90067-6.
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