Davison J, Brunel F, Merchez M, Ha-Thi V
Gene. 1981 Dec;16(1-3):99-106. doi: 10.1016/0378-1119(81)90066-4.
The adjacent PstI-J, I and G fragments of the phage T5 DNA molecule (4.4, 4.6 and 7.2 kb, respectively) have been cloned in plasmid pBR322 and their locations verified by Southern blot analysis. The PstI I and G fragments overlap the previously cloned HindIII-P and G fragments and like those, contain no known genetic markers. In addition, one of the 12 newly isolated T5 mutants maps in this PstI-IG region. Thus, the size of the "empty" region between genes D15 and D17, which we have previously observed on the genetic map, extends to at least 11.8 kb. In contrast, the PstI-J fragment carried part of the D12 gene and the intact D14 and D15 genes. This clone is of particular interest since the D15 gene product is a nuclease and is responsible for the positive control of late gene transcription. The orientation of these genes relative to the T5 DNA molecule has been determined by a combination of restriction, deletion and complementation analyses.
噬菌体T5 DNA分子相邻的PstI-J、I和G片段（分别为4.4、4.6和7.2 kb）已克隆到质粒pBR322中，并通过Southern印迹分析验证了它们的位置。PstI I和G片段与先前克隆的HindIII-P和G片段重叠，并且与那些片段一样，不包含已知的遗传标记。此外，新分离的12个T5突变体之一定位在这个PstI-IG区域。因此，我们先前在遗传图谱上观察到的基因D15和D17之间“空白”区域的大小至少延伸到11.8 kb。相比之下，PstI-J片段携带了部分D12基因以及完整的D14和D15基因。这个克隆特别令人感兴趣，因为D15基因产物是一种核酸酶，负责晚期基因转录的正调控。通过限制性酶切、缺失和互补分析相结合的方法确定了这些基因相对于T5 DNA分子的方向。
