Boothroyd J C, Harris T J, Rowlands D J, Lowe P A
Gene. 1982 Feb;17(2):153-61. doi: 10.1016/0378-1119(82)90068-3.
The complete nucleotide sequence of cDNA coding for the structural capsid polypeptides of foot-and-mouth disease virus (FMDV) (strain A(10)61) has been determined. Portions of the flanking sequence coding for the nonstructural proteins p20a and p52 are also provided. The three larger structural polypeptides VP1, VP2 and VP3 have unmodified Mrs of 23248, 24649 and 24213, respectively. The size of the smaller polypeptide, VP4, can only be estimated at 7360 because the 5'-limit of its coding region is not yet known with certainty. The sequence data for VP1 (the major immunising antigen) and the amino-terminal quarter of p52 are compared with the data of Kurz et al. (Nucl. Acids Res. 9 (1981) 1919-1931) for a different serotype (O1K). This shows that variation is much greater in the region coding for VP1 than in that coding for p52. This is reflected in the level of amino acid sequence variation predicted for the two proteins. Analysis of relative codon usage reveals a strong bias in favour of C and G over U and A in the third base position. The dinucleotide frequencies show a bias against A-U and U-A, and for A-C and C-A.
已确定口蹄疫病毒（FMDV）（A（10）61株）结构衣壳多肽编码cDNA的完整核苷酸序列。还提供了编码非结构蛋白p20a和p52的侧翼序列部分。三种较大的结构多肽VP1、VP2和VP3的未修饰分子量分别为23248、24649和24213。较小的多肽VP4的大小只能估计为7360，因为其编码区的5'端尚未确定。将VP1（主要免疫抗原）和p52氨基末端四分之一的序列数据与Kurz等人（《核酸研究》9（1981）1919 - 1931）针对不同血清型（O1K）的数据进行了比较。这表明编码VP1的区域的变异比编码p52的区域大得多。这反映在预测的两种蛋白质的氨基酸序列变异水平上。相对密码子使用情况分析显示，在第三个碱基位置上，强烈偏向于C和G而非U和A。二核苷酸频率显示出对A - U和U - A的偏向，以及对A - C和C - A的偏向。
