Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen.
EMBO J. 1984 Apr;3(4):855-61. doi: 10.1002/j.1460-2075.1984.tb01896.x.
Computer analyses have revealed sequence homology between two non-structural proteins encoded by cowpea mosaic virus (CPMV), and corresponding proteins encoded by two picornaviruses, poliovirus and foot-and-mouth disease virus. A region of 535 amino acids in the 87-K polypeptide from CPMV was found to be homologous to the RNA-dependent RNA polymerases from both picornaviruses, the best matches being found where the picornaviral proteins most resemble each other. Additionally, the 58-K polypeptide from CPMV and polypeptide P2-X from poliovirus contain a conserved region of 143 amino acids. Based on the homology observed, a genetic map of the CPMV genome has been constructed in which the 87-K polypeptide represents the core polymerase domain of the CPMV replicase. These results have implications for the evolution of RNA viruses, and mechanisms are discussed which may explain the existence of homology between picornaviruses (animal viruses with single genomic RNAs) and comoviruses (plant viruses with two genomic RNAs).
计算机分析表明豇豆花叶病毒（CPMV）两个非结构蛋白编码序列与两个小核糖核酸病毒（小 RNA 病毒），脊髓灰质炎病毒和口蹄疫病毒相应蛋白编码序列具有同源性。在 CPMV 的 87-K 多肽中，发现有 535 个氨基酸的区域与来自两种小 RNA 病毒的 RNA 依赖性 RNA 聚合酶具有同源性，在小 RNA 病毒蛋白彼此最相似的地方找到了最佳匹配。此外，CPMV 的 58-K 多肽和脊髓灰质炎病毒 P2-X 多肽含有 143 个氨基酸的保守区域。基于观察到的同源性，构建了 CPMV 基因组的遗传图谱，其中 87-K 多肽代表 CPMV 复制酶的核心聚合酶结构域。这些结果对 RNA 病毒的进化具有重要意义，并讨论了可能解释小 RNA 病毒（具有单基因组 RNA 的动物病毒）和伴随 RNA 病毒（具有两个基因组 RNA 的植物病毒）之间同源性存在的机制。
