Biswas G D, Graves J F, Sox T E, Tenover F C, Sparling P F
J Bacteriol. 1982 Jul;151(1):77-82. doi: 10.1128/jb.151.1.77-82.1982.
A 42-kilobase hybrid Pcr plasmid (pFA14) was formed when the naturally occurring 7.2-kilobase Pcr plasmid pFA3 was introduced by transformation into a competent gonococcal recipient containing the 36-kilobase conjugative plasmid pFA2 (Sox et al., J. Bacteriol. 138:510-518). Analysis of the structure of pFA14 showed that it was a stable recombinant between pFA3 and pFA2. The transformation efficiency of pFA14 was increased 300- to 10,000-fold by the presence in isogenic recipients of the homologous plasmid pFA2. The presence of a homologous plasmid in the recipient also markedly increased the likelihood of recovery of intact donor-size Pcr plasmids in the transformants. The presence of pFA2 had no effect on the competence of piliated or nonpiliated gonococci for transformation by either linear chromosomal DNA or a nonhomologous Pcr plasmid. Increased transformation efficiency of the hybrid Pcr plasmid pFA14 may have been due to recombination between the nicked or linearized donor plasmid and the homologous recipient plasmid (marker rescue).
当天然存在的7.2千碱基的Pcr质粒pFA3通过转化导入含有36千碱基接合质粒pFA2的感受态淋球菌受体时，形成了一个42千碱基的杂交Pcr质粒（pFA14）（索克斯等人，《细菌学杂志》138:510 - 518）。对pFA14结构的分析表明，它是pFA3和pFA2之间的稳定重组体。在同基因受体中存在同源质粒pFA2时，pFA14的转化效率提高了300至10000倍。受体中同源质粒的存在也显著增加了在转化体中回收完整供体大小Pcr质粒的可能性。pFA2的存在对有菌毛或无菌毛淋球菌通过线性染色体DNA或非同源Pcr质粒进行转化的感受态没有影响。杂交Pcr质粒pFA14转化效率的提高可能是由于切口或线性化的供体质粒与同源受体质粒之间的重组（标记拯救）。
