Meyers J A, Sanchez D, Elwell L P, Falkow S
J Bacteriol. 1976 Sep;127(3):1529-37. doi: 10.1128/jb.127.3.1529-1537.1976.
Agarose gel electrophoresis may be employed effectively for the detection and preliminary characterization of plasmid deoxyribonucleic acid (DNA) present in clinical isolates and laboratory strains of gram-negative microorganisms. The method is sensitive and does not require radioisotopes or ultracentrifugation. The estimation of plasmid mass from the extent of DNA migration in gels compares favorably with results obtained by electron microscopy of plasmid DNA purified by equilibrium density centrifugation. The method has proved to be a useful tool for survey work and the epidemiological investigation of plasmid dissemination, as well as an important adjunct to the genetic analysis of plasmids.
琼脂糖凝胶电泳可有效地用于检测革兰氏阴性微生物临床分离株和实验室菌株中存在的质粒脱氧核糖核酸（DNA）并进行初步鉴定。该方法灵敏，且不需要放射性同位素或超速离心。根据凝胶中DNA迁移程度估算质粒质量，与通过平衡密度离心法纯化的质粒DNA进行电子显微镜观察所得到的结果相比毫不逊色。该方法已被证明是用于调查工作和质粒传播的流行病学研究的有用工具，也是质粒遗传分析的重要辅助手段。
