Murray J D, Bellett A J, Braithwaite A w, Waldron L K, Taylor I W
J Cell Physiol. 1982 Apr;111(1):89-96. doi: 10.1002/jcp.1041110114.
Actively growing mouse or rat embryo cells suffered structural chromosome damage, mitotic anomalies, and polyploidy after infection by human adenovirus type 5. Chromosome damage required expression of one or more early viral genes and showed regular periodicity in its frequency. The growth cycle time of some of the infected cells was reduced by about 5 hours due to a decrease in G1, and the interval between successive waves of chromosome damage corresponded to this reduced cycle time. After infection there was a decrease in cells with G1 DNA content and an increase in cells with G2 diploid, aneuploid, and polyploid DNA contents. We suggest these effects are due to the expression in semipermissive cells fo early viral gene(s), whose function in productive infection in vivo is to alter cell cycle controls in order to maximize the number of cells able to replicate viral DNA and the time such cells spend in DNA replication.
感染5型人腺病毒后，处于活跃生长状态的小鼠或大鼠胚胎细胞会出现染色体结构损伤、有丝分裂异常和多倍体现象。染色体损伤需要一个或多个早期病毒基因的表达，并且其频率呈现出规律的周期性。由于G1期缩短，一些受感染细胞的生长周期时间减少了约5小时，连续几波染色体损伤之间的间隔与这种缩短的周期时间相对应。感染后，G1期DNA含量的细胞减少，而G2期二倍体、非整倍体和多倍体DNA含量的细胞增加。我们认为，这些效应是由于早期病毒基因在半允许细胞中的表达所致，这些基因在体内生产性感染中的功能是改变细胞周期调控，以最大限度地增加能够复制病毒DNA的细胞数量以及这些细胞在DNA复制中所花费的时间。
