腺病毒诱导的细胞生长周期改变：早期区域E2A和E2B突变的影响
Adenovirus-induced alterations of the cell growth cycle: effects of mutations in early regions E2A and E2B.
作者信息
Murray J D, Braithwaite A W, Taylor I W, Bellett A J
出版信息
J Virol. 1982 Dec;44(3):1072-5. doi: 10.1128/JVI.44.3.1072-1075.1982.
Abstract
Mutants ts125 (E2A) and ts36 (E2B) of adenovirus type 5 induced alterations to cell cycle progression at the nonpermissive temperature which were detectable by flow cytometry. Thus neither E2A, nor gene N in E2B, is required for these effects. Whereas the wild-type virus induced cells with aneuploid (between 4n and 8n) DNA contents, as did ts125 at the permissive temperature, ts125 induced peaks of cells with 8n, 16n, and 32n DNA contents at the nonpermissive temperature. This was probably due to the failure of regulation of E1A by E2.
摘要
5型腺病毒的突变体ts125（E2A）和ts36（E2B）在非允许温度下诱导细胞周期进程发生改变，这种改变可通过流式细胞术检测到。因此，这些效应既不需要E2A，也不需要E2B中的基因N。野生型病毒诱导细胞具有非整倍体（4n至8n之间）的DNA含量，ts125在允许温度下也是如此，但ts125在非允许温度下诱导出DNA含量为8n、16n和32n的细胞峰。这可能是由于E2对E1A的调节失败所致。
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