神经母细胞瘤X胶质瘤杂交细胞中锂离子通过动作电位钠通道的通量的药理学和电生理学特征

Pharmacological and electrophysiological characterization of lithium ion flux through the action potential sodium channel in neuroblastoma X glioma hybrid cells.

作者信息

Reiser G, Scholz F, Hamprecht B

出版信息

J Neurochem. 1982 Jul;39(1):228-34. doi: 10.1111/j.1471-4159.1982.tb04723.x.

DOI:10.1111/j.1471-4159.1982.tb04723.x

Abstract

Interaction of Li+ with the voltage-dependent Na+ channel has been analyzed in neuroblastoma X glioma hybrid cells. The cells were able to generate action potentials in media containing Li+ instead of Na+. The uptake of Li+ into the hybrid cells was investigated for the pharmacological analysis of Li+ permeation through voltage-dependent Na+ channels. Veratridine and aconitine increased the uptake of Li+ to the same degree (EC50 30 microM). This increase was blocked by tetrodotoxin (IC50 20 nM). Veratridine and aconitine did not act synergistically; however, the veratridine-stimulated influx was further enhanced by the toxin of the scorpion Leiurus quinquestriatus (EC50 0.06 micrograms/ml). This stimulation was also blocked by tetrodotoxin. Thus, the voltage-dependent Na+ channel of the hybrid cells accepts both Li+ and Na+ in a similar manner.

摘要

已在神经母细胞瘤X胶质瘤杂交细胞中分析了Li⁺与电压依赖性Na⁺通道的相互作用。这些细胞能够在含有Li⁺而非Na⁺的培养基中产生动作电位。为了对Li⁺通过电压依赖性Na⁺通道的渗透进行药理学分析，研究了Li⁺进入杂交细胞的摄取情况。藜芦碱和乌头碱使Li⁺摄取增加到相同程度（半数有效浓度30微摩尔）。这种增加被河豚毒素阻断（半数抑制浓度20纳摩尔）。藜芦碱和乌头碱没有协同作用；然而，蝎毒Leiurus quinquestriatus的毒素进一步增强了藜芦碱刺激的内流（半数有效浓度0.06微克/毫升）。这种刺激也被河豚毒素阻断。因此，杂交细胞的电压依赖性Na⁺通道以类似方式接受Li⁺和Na⁺。