Gilbert F, Giovanni M Y, Silver I A, Glick M C
Department of Pediatrics, Mt. Sinai School of Medicine, NY 10029.
FEBS Lett. 1988 Aug 15;236(1):39-42. doi: 10.1016/0014-5793(88)80281-3.
The voltage-sensitive Na+ channel is responsible for the action potential of membrane electrical excitability in neuronal tissue. Three methods were used to demonstrate the presence of neurotoxin-responsive Na+ channels in two hybrid cell lines resulting from the fusion of excitable human neuroblastoma cells with mouse fibroblasts. Only one of the two electrically active hybrid cell lines maintained the sensitivity of the neuroblastoma parent to tetrodotoxin (TTX). The other hybrid, although electrically active, was not responsive to TTX or scorpion venom. Comparisons of the patterns of expression of membrane excitability and of chromosome complements in these human neuroblastoma cell hybrids suggest that the phenotype of membrane excitability is composed of genetically distinct elements.
电压敏感钠通道负责神经元组织中膜电兴奋性的动作电位。采用三种方法来证明在由可兴奋的人神经母细胞瘤细胞与小鼠成纤维细胞融合产生的两种杂交细胞系中存在神经毒素反应性钠通道。两个电活性杂交细胞系中只有一个保持了神经母细胞瘤亲本对河豚毒素（TTX）的敏感性。另一个杂交细胞虽然有电活性，但对TTX或蝎毒无反应。对这些人神经母细胞瘤细胞杂交体中膜兴奋性表达模式和染色体互补情况的比较表明，膜兴奋性表型由基因上不同的元件组成。
