Swanstrom R, Bishop J M, Varmus H E
J Virol. 1982 Apr;42(1):337-41. doi: 10.1128/JVI.42.1.337-341.1982.
Intermediates in the synthesis of Rous sarcoma virus DNA in vivo contain a short second strand of DNA (plus strong-stop DNA) synthesized by using the region near the 5' end of the first (minus) strand of DNA as the template. In this report, we show that the 3' end of plus strong-stop DNA is extended about 15 to 20 nucleotides beyond the 5' end of the minus-strand DNA template, probably copying a portion of the tRNATrp molecule that serves as primer for synthesis of the minus strand of DNA. The extra sequences present in plus strong-stop DNA may play a central role in the generation of the long terminal repeat present in mature forms of viral DNA.
劳氏肉瘤病毒DNA在体内合成过程中的中间体包含一条短的第二条DNA链（加上强终止DNA），它是以第一条（负）DNA链5'端附近区域为模板合成的。在本报告中，我们表明，正链强终止DNA的3'端比负链DNA模板的5'端延伸约15至20个核苷酸，可能复制了一部分用作负链DNA合成引物的tRNATrp分子。正链强终止DNA中存在的额外序列可能在成熟形式的病毒DNA中存在的长末端重复序列的产生中起核心作用。
