Clayman C H, Mosharrafa E, Faras A J
J Virol. 1979 Jan;29(1):242-9. doi: 10.1128/JVI.29.1.242-249.1979.
Infectious DNA molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the Rous sarcoma virus-associated reverse transcriptase in vitro. The optimal enzymatic conditions employed for infectious DNA synthesis also facilitate maximum synthesis of genome length DNA. Analysis of the DNA product synthesized by detergent-disrupted Rous sarcoma virus under these conditions indicates that DNA complementary to viral RNA (minus-strand DNA) is genome length in size, whereas DNA complementary to genome length minus-strand DNA (plus-strand DNA) appears as subgenomic-length molecules ranging between 300 and 3,500 nucleotides in length. These features of the DNA product synthesized by the Rous sarcoma virus reverse transcriptase in vitro are similar to those identified in the cytoplasm of cells shortly after infection and lend credence to studies of the mechanism of reverse transcription in vitro and their significance to proviral DNA synthesis in vivo.
能够转化鸡胚成纤维细胞的传染性DNA分子，可以由劳氏肉瘤病毒相关的逆转录酶在体外合成。用于传染性DNA合成的最佳酶促条件也有利于基因组长度DNA的最大合成。在这些条件下对经去污剂处理的劳氏肉瘤病毒合成的DNA产物进行分析表明，与病毒RNA互补的DNA（负链DNA）在大小上是基因组长度，而与基因组长度负链DNA互补的DNA（正链DNA）则表现为亚基因组长度的分子，长度在300至3500个核苷酸之间。劳氏肉瘤病毒逆转录酶在体外合成的DNA产物的这些特征与感染后不久在细胞细胞质中鉴定出的特征相似，这为体外逆转录机制的研究及其对体内前病毒DNA合成的意义提供了可信度。
