促肾上腺皮质激素释放因子（CRF）：对体外培养的人前叶垂体细胞释放阿片促黑皮质素原（POMC）相关肽的影响

Corticotropin releasing factor (CRF): effects on the release of pro-opiomelanocortin (POMC)-related peptides by human anterior pituitary cells in vitro.

作者信息

Chan J S, Lu C L, Seidah N G, Chretien M

出版信息

Endocrinology. 1982 Oct;111(4):1388-90. doi: 10.1210/endo-111-4-1388.

Abstract

The effect of synthetic ovine corticotropin-releasing factor (oCRF), alpha-melanotropin (alpha-MSH) and arginine-vasopressin (AVP) on the release of pro-opiomelanocortin (POMC)-related peptides (beta-endorphin, beta-lipotropin, adrenocorticotropin, and the 12K N-terminal) was studied in primary cultures of human anterior pituitary (HAP) cells. The peptides released into the medium were measured by specific radioimmunoassays. HAP cells responded to oCRF at concentrations as low as 10 ng/ml by significantly increasing peptide secretion (p less than 0.005). AVP was also effective, but, it is only 0.1% as potent as oCRF. alpha-MSH was ineffective even at a dose of 10 micrograms/ml. Immunoreactive (IR)-beta EP, IR-beta LPH, IR-ACTH, and IR-HNT were released in approximately equimolar ratios. Partial characterization of the peptides released by HAP cells with gel chromatography on Sephadex G-50 superfine revealed two peaks of IR-beta EP (as beta EP and beta-LPH), two peaks of IR-beta LPH (as beta-LPH and gamma-LPH), one peak of IR-ACTH, and two peaks of IR-HNT. In conclusion, oCRF is a potent secretagogue for the equimolar release of ACTH, HNT and related peptides in the human pituitary gland.

摘要

在人垂体前叶（HAP）细胞的原代培养物中，研究了合成的羊促肾上腺皮质激素释放因子（oCRF）、α-促黑素（α-MSH）和精氨酸加压素（AVP）对阿片-促黑素原（POMC）相关肽（β-内啡肽、β-促脂素、促肾上腺皮质激素和12K N端肽）释放的影响。通过特异性放射免疫测定法测量释放到培养基中的肽。HAP细胞对低至10 ng/ml浓度的oCRF有反应，肽分泌显著增加（p<0.005）。AVP也有效，但效力仅为oCRF的0.1%。即使在10μg/ml的剂量下，α-MSH也无效。免疫反应性（IR）-β-内啡肽、IR-β-促脂素、IR-促肾上腺皮质激素和IR-HNT以近似等摩尔比释放。用Sephadex G-50超细凝胶色谱法对HAP细胞释放的肽进行部分表征，发现IR-β-内啡肽有两个峰（分别为β-内啡肽和β-促脂素），IR-β-促脂素有两个峰（分别为β-促脂素和γ-促脂素），IR-促肾上腺皮质激素有一个峰，IR-HNT有两个峰。总之，oCRF是一种有效的促分泌剂，可使人垂体中促肾上腺皮质激素、HNT及相关肽等摩尔释放。