Vial H J, Thuet M J, Philippot J R
Mol Biochem Parasitol. 1982 Mar;5(3):189-98. doi: 10.1016/0166-6851(82)90020-2.
D vitamins are effective inhibitors of the in vitro intraerythrocytic growth of Plasmodium falciparum. Disappearance of the parasitemia was observed after 48 h contact between infected cells and 5 x 10(-6) M 1 alpha-hydroxycholecalciferol, 5 x 10(-5) M 25-hydroxycholecalciferol (25-OH-D-3), 1 alpha, 25-dihydroxycholecalciferol or 2.5 x 10(-4) vitamin D-2 and D-3. A 48 h pretreatment of healthy erythrocytes with 5 x 10(-5) M 25-OH-D-3 did not change their susceptibility to invasion by the parasite and their ability to support the growth of P. falciparum. Ionomycin, a calcium ionophore, and EGTA prevented parasite development at concentrations greater than 2 x 10(-7) M and 4 x 10(-4) M, respectively, but did not antagonize the inhibitory activity of 25-OH-D-3. Addition of 25-OH-D-3 for 12 or 24 h duration to synchronized cultures, showed that the drug had a schizonticidal action, but was without effect when parasites were in the ring form.
D族维生素是恶性疟原虫体外红细胞内生长的有效抑制剂。在感染细胞与5×10⁻⁶M 1α-羟基胆钙化醇、5×10⁻⁵M 25-羟基胆钙化醇（25-OH-D-3）、1α,25-二羟基胆钙化醇或2.5×10⁻⁴维生素D-2和D-3接触48小时后，观察到疟原虫血症消失。用5×10⁻⁵M 25-OH-D-3对健康红细胞进行48小时预处理，并未改变其对寄生虫侵袭的敏感性及其支持恶性疟原虫生长的能力。离子霉素（一种钙离子载体）和乙二醇双四乙酸（EGTA）分别在浓度大于2×10⁻⁷M和4×10⁻⁴M时可阻止寄生虫发育，但不拮抗25-OH-D-3的抑制活性。在同步培养物中添加25-OH-D-3持续12或24小时，结果表明该药物具有裂殖体杀灭作用，但当寄生虫处于环状体形式时则无作用。
