Vasilenko T F, Pisareva L N, Skvortsevich E G
Tsitologiia. 1980 Jan;22(1):44-51.
The effect of surface active agents on the activity of Na, K-ATPase and on the direct medium 18O exchange in the membrane preparations of the guinea pig kidney has been studied. The medium 18O exchange was considered as a character of the K+-dependent stage in ATPase reaction. Low concentration of all the surface active agents were shown to stimulate, and high concentrations -- to inhibit both ATPase and medium 18O exchange. The relation between medium 18O exchange and Na,K-ATPase activity was found to be equal to 1.5 +/- 0.1. The treatment of preparations by activating amounts of the surface active agents resulted in lowering this relation up to 1.0 +/- 0.09 and 1.3 +/- 0.04 for DOC and triton X-100, correspondently, due to a stronger stimulation of ATPase activity than of medium 18O exchange. With the inhibiting amounts of triton X-100 and histone H2a, this relation did not change, but it decreased in the presence of equal amounts of DOC.
研究了表面活性剂对豚鼠肾膜制剂中钠钾ATP酶活性及介质18O直接交换的影响。介质18O交换被视为ATP酶反应中钾离子依赖阶段的一个特征。结果表明，所有表面活性剂的低浓度均能刺激钠钾ATP酶和介质18O交换，而高浓度则起抑制作用。介质18O交换与钠钾ATP酶活性之间的关系为1.5±0.1。用激活量的表面活性剂处理制剂后，由于对ATP酶活性的刺激强于对介质18O交换的刺激，该关系分别降至1.0±0.09（去氧胆酸钠）和1.3±0.04（曲拉通X-100）。使用抑制量的曲拉通X-100和组蛋白H2a时，该关系不变，但在等量去氧胆酸钠存在时关系降低。
