Anekwe G E
Afr J Med Med Sci. 1980 Sep-Dec;9(3-4):103-9.
Deoxyribonucleic acid (DNA) extracted from canine venereal tumours was capable of changing normal cells to morphologically abnormal cells in an in vitro system of L-fibroblasts. The observed effects were proportional to the concentration of the nucleic acid extract used; the higher the concentration, the more pronounced the effect. Inactivation of this effect by deoxyribonuclease indicated that the active nucleic acid moiety was DNA. Purified RNA did not appear to contribute to the observed activity. The activity was enhanced by diethyl-amino ethyl-dextran (DEAE-D) and dimethyl sulphoxide (DMSO) and the data showed that 20% DMSO was superior to 300 g/ml DEAE-D as an enhancing agent.
从犬类性病肿瘤中提取的脱氧核糖核酸（DNA），在L-成纤维细胞的体外系统中能够将正常细胞转变为形态异常的细胞。观察到的效应与所用核酸提取物的浓度成正比；浓度越高，效应越明显。脱氧核糖核酸酶使这种效应失活，表明活性核酸部分是DNA。纯化的RNA似乎对观察到的活性没有贡献。二乙氨基乙基葡聚糖（DEAE-D）和二甲基亚砜（DMSO）可增强该活性，数据表明，作为增强剂，20%的DMSO比300微克/毫升的DEAE-D更具优势。
