Massari S
Biochim Biophys Acta. 1982 May 21;688(1):23-8. doi: 10.1016/0005-2736(82)90574-0.
The gel to liquid-crystalline phase transition of aqueous dispersions of phospholipid mixtures was investigated by means of the repartition of the spin label 2,2,6,6-tetramethylpiperidine-I-oxyl between aqueous space and lipid hydrocarbon region. The dimyristoylphosphatidylcholine (DMPC)/dibehenoylphosphatidylcholine (DBPC) and dipalmitoylphosphatidylcholine (DPPC)/DBPC phase diagrams indicate gel phase immiscibility, whereas the distearoylphosphatidylcholine (DSPC)/DBPC phase diagram indicates non-ideal gel phase miscibility at low DBPC molar fractions. Aqueous dispersions of DMPC/DPPC/DBPC ternary mixtures show two distinct phase transitions, the first associated with the melting of a DMPC/DPPC phase and the second with the melting of a DBPC phase. Aqueous dispersions of DMPC/DSPC/DBPC ternary mixtures show to phase transitions at low DSPC molar fractions; the first is probably associated with the melting of a DMPC/DSPC phase, and the second with the melting of a DBPC/DSPC phase. At high DSPC molar fractions, only one phase transition is observed; this suggests that all the lipids are mixed in gel state membranes.
通过自旋标记物2,2,6,6 - 四甲基哌啶 - 1 - 氧基在水相空间和脂质烃区域之间的重新分布，研究了磷脂混合物水分散体从凝胶到液晶相的转变。二肉豆蔻酰磷脂酰胆碱（DMPC）/二山嵛酰磷脂酰胆碱（DBPC）和二棕榈酰磷脂酰胆碱（DPPC）/DBPC的相图表明凝胶相不相容，而硬脂酰磷脂酰胆碱（DSPC）/DBPC的相图表明在低DBPC摩尔分数下为非理想凝胶相混溶。DMPC/DPPC/DBPC三元混合物的水分散体显示出两个不同的相变，第一个与DMPC/DPPC相的熔化有关，第二个与DBPC相的熔化有关。DMPC/DSPC/DBPC三元混合物的水分散体在低DSPC摩尔分数下显示出两个相变；第一个可能与DMPC/DSPC相的熔化有关，第二个与DBPC/DSPC相的熔化有关。在高DSPC摩尔分数下，仅观察到一个相变；这表明所有脂质在凝胶态膜中混合。
