甲基化检测中不同DNA回收方法的比较。

Comparison of different methods of recovering DNA from a methylation assay.

作者信息

Pfohl-Leszkowicz A, Dirheimer G

出版信息

Biochimie. 1982 Apr;64(4):293-6. doi: 10.1016/s0300-9084(82)80498-7.

Abstract

Several enzymes, for example DNA (cytosine-5-)-methyltransferase, produce relatively strong interactions with DNA and hinder the quantitative recovery of this DNA from a reaction mixture. Classical methods like the Sevag chloroform-iso-amylic alcohol one or the phenol procedure lead only to a 50-60 per cent recovery of the DNA. A new procedure was worked out utilizing pancreatic RNase, proteinase K, NaOH 0.5 M treatment and trichloracetic acid precipitation which gives 85 per cent recovery of DNA.

摘要

几种酶，例如DNA（胞嘧啶-5-）-甲基转移酶，与DNA产生相对较强的相互作用，并阻碍从反应混合物中定量回收这种DNA。像Sevag氯仿-异戊醇法或苯酚法这样的经典方法只能使DNA的回收率达到50%-60%。一种新方法已经制定出来，该方法利用胰核糖核酸酶、蛋白酶K、0.5M氢氧化钠处理和三氯乙酸沉淀，可使DNA的回收率达到85%。