延迟甲基化与基质结合DNA甲基化酶
Delayed methylation and the matrix bound DNA methylase.
作者信息
Davis T, Kirk D, Rinaldi A, Burdon R H, Adams R L
出版信息
Biochem Biophys Res Commun. 1985 Jan 31;126(2):678-84. doi: 10.1016/0006-291x(85)90238-4.
PMID:2579643
Abstract
It is shown that the methylation of DNA that occurs in isolated nuclei is "delayed methylation". This methylation is not reduced in nuclei which have been pretreated with 0.2M NaCl to extract the soluble methylase suggesting that this methylation is the product of a firmly bound matrix associated DNA methylase. Evidence is provided that, like the methylase, the DNA substrate is associated with the nuclear matrix.
摘要
结果表明，在分离的细胞核中发生的DNA甲基化是“延迟甲基化”。在用0.2M NaCl预处理以提取可溶性甲基化酶的细胞核中，这种甲基化并没有减少，这表明这种甲基化是与核基质紧密结合的DNA甲基化酶的产物。有证据表明，与甲基化酶一样，DNA底物也与核基质相关。
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