细胞色素c与铁氰化物之间电子交换的1H核磁共振研究。细胞色素c上铁氰化物结合位点的定义。

1H NMR studies of the electron exchange between cytochrome c and iron hexacyanides. Definition of the iron hexacyanide binding sites on cytochrome c.

作者信息

Eley C G, Moore G R, Williams G, Williams R J

出版信息

Eur J Biochem. 1982 May 17;124(2):295-303. doi: 10.1111/j.1432-1033.1982.tb06591.x.

DOI:10.1111/j.1432-1033.1982.tb06591.x

Abstract

Binding of [Fe(CN)6]3-, [Cr(CN)6]3-, [Co(CN)6]3- and [Cr(C2O4)3]3- to horse, tuna and Candida krusei cytochromes c has been studied by high-resolution 1H NMR spectroscopy. All the reagents bind at the same sites. There are at least two binding sites, and probably three, on horse cytochrome c at pH 7. One of the sites is only a weak binding site and is far from the haem group, whereas the other site(s) is(are) at the haem crevice. Ka for binding of [Fe(CN)6]3- to trimethyllysine-72 of C. krusei ferricytochrome c is 140 +/- 15 M-1 at 27 degrees C and pH 7.

摘要

通过高分辨率1H核磁共振光谱研究了[Fe(CN)6]3-、[Cr(CN)6]3-、[Co(CN)6]3-和[Cr(C2O4)3]3-与马、金枪鱼和克鲁斯假丝酵母细胞色素c的结合情况。所有试剂都结合在相同的位点上。在pH 7时，马细胞色素c上至少有两个结合位点，可能有三个。其中一个位点只是一个弱结合位点，远离血红素基团，而其他位点位于血红素裂隙处。在27℃和pH 7条件下，[Fe(CN)6]3-与克鲁斯假丝酵母高铁细胞色素c的三甲基赖氨酸-72结合的Ka为140±15 M-1。