细胞色素c与细胞色素b5相互作用的1H核磁共振研究
1H-n.m.r. investigation of the interaction between cytochrome c and cytochrome b5.
作者信息
Eley C G, Moore G R
出版信息
Biochem J. 1983 Oct 1;215(1):11-21. doi: 10.1042/bj2150011.
Abstract
The interaction between eukaryotic cytochrome c and the tryptic fragment of bovine liver microsomal cytochrome b5 was studied by 1H-n.m.r. spectroscopy, and a procedure was developed that may be generally applicable to the study of macromolecular interactions by n.m.r. At pH6.3 (27 degrees C, I approx. 0.04) the two ferricytochromes were found to form a 1:1 complex with an association constant of approx. 10(3) M -1. The protein--protein-interaction region was found to encompass the region of the surface of horse cytochrome c that includes Ile-81, Phe-82, Ala-83 and Ile-85, and Lys-13 and Lys-72 of horse cytochrome c were suggested to be involved in two important intermolecular interactions. Me3Lys-72 of Candida krusei cytochrome c was shown to be involved in the interaction.
摘要
通过核磁共振光谱研究了真核细胞色素c与牛肝微粒体细胞色素b5胰蛋白酶片段之间的相互作用，并开发了一种程序，该程序可能普遍适用于通过核磁共振研究大分子相互作用。在pH6.3（27℃，离子强度约为0.04）时，发现两种高铁细胞色素形成1:1复合物，缔合常数约为10³M⁻¹。发现蛋白质-蛋白质相互作用区域包括马细胞色素c表面的区域，该区域包括Ile-81、Phe-82、Ala-83和Ile-85，并且推测马细胞色素c的Lys-13和Lys-72参与两种重要的分子间相互作用。克鲁斯假丝酵母细胞色素c的Me3Lys-72被证明参与了这种相互作用。
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