Berlan M, Carpene C, Lafontan M, Dang-Tran L
Horm Metab Res. 1982 May;14(5):257-60. doi: 10.1055/s-2007-1018986.
The main intention of this study was to characterize the alpha-adrenoceptor responsible for the inhibition of lipolysis in dog fat cell and to define circumstances that may be associated to a modification of the alpha-mediated antilipolytic effect. Isolated fat cells from omental and subcutaneous adipose tissue from normal and obese dogs were used. Basal and theophylline stimulated lipolysis was studied in the presence of selected alpha-adrenergic agonists and antagonists. The antilipolytic effect of catecholamines is mediated by alpha 2-type adrenoceptors in dog fat cell. The alpha-adrenergic responsiveness is enhanced (or unmasked) in large fat cells of obese dogs and depends on the site from which the adipose tissue sample is taken. The alpha-response is stronger in subcutaneous than in omental adipocytes. In conclusion, the weakened lipolytic responsiveness to epinephrine of obese dog fat cells seems related to an increased alpha-adrenergic response rather than a decreased beta-lipolytic effect. Obesity is a circumstance characterized in the dog fat cell by a modification of the balance between alpha-2 and beta receptors.
本研究的主要目的是鉴定犬脂肪细胞中负责抑制脂肪分解的α-肾上腺素能受体，并确定可能与α介导的抗脂解作用改变相关的情况。使用了来自正常和肥胖犬网膜及皮下脂肪组织的分离脂肪细胞。在选定的α-肾上腺素能激动剂和拮抗剂存在的情况下，研究了基础和茶碱刺激的脂肪分解。儿茶酚胺的抗脂解作用由犬脂肪细胞中的α2型肾上腺素能受体介导。肥胖犬的大脂肪细胞中α-肾上腺素能反应性增强（或被揭示），且取决于脂肪组织样本的取材部位。皮下脂肪细胞的α反应比网膜脂肪细胞更强。总之，肥胖犬脂肪细胞对肾上腺素的脂解反应性减弱似乎与α-肾上腺素能反应增加有关，而非β-脂解作用降低。肥胖是犬脂肪细胞中α-2和β受体之间平衡改变的一种情况。
