Suppr超能文献

使用激动剂UK 14304证明成年大鼠脂肪细胞中存在功能性α2-肾上腺素能受体。

Evidence of functional alpha 2-adrenergic receptors in adult-rat adipocytes by using the agonist UK 14304.

作者信息

Rebourcet M C, Carpéné C, Lavau M

机构信息

INSERM Unité 177, Paris, France.

出版信息

Biochem J. 1988 Jun 15;252(3):679-82. doi: 10.1042/bj2520679.

DOI:10.1042/bj2520679
PMID:2901830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149202/
Abstract

The aim of this study was to re-assess whether alpha 2-adrenergic receptors were present in rat adipocytes, by using UK 14304, a new and very selective alpha 2-agonist. The following observations demonstrate the presence of functional alpha 2-adrenoceptors in rat adipocytes. (1) Adipocyte lipolysis was dose-dependently inhibited by UK 14304 (maximal effect 80% at 1 microM-UK 14304, 45% at 10 microM-UK 14304, under basal or theophylline-stimulated conditions respectively). (2) UK 14304 bound specifically to purified plasma membranes, with Bmax. = 744 fmol/mg of protein and KD = 9 nM. (3) The effect of UK 14304 was suppressed by alpha 2-antagonists. (4) Adrenaline inhibited lipolysis upon beta-adrenergic blockade (propranolol). (5) The anti-lipolytic effect of UK 14304 was modulated by the age of the rats.

摘要

本研究的目的是通过使用新型且极具选择性的α2-激动剂UK 14304，重新评估大鼠脂肪细胞中是否存在α2-肾上腺素能受体。以下观察结果证明大鼠脂肪细胞中存在功能性α2-肾上腺素能受体。（1）UK 14304剂量依赖性地抑制脂肪细胞脂解（在基础或茶碱刺激条件下，最大效应分别为：1 μM UK 14304时为80%，10 μM UK 14304时为45%）。（2）UK 14304与纯化的质膜特异性结合，Bmax. = 744 fmol/mg蛋白质，KD = 9 nM。（3）UK 14304的作用被α2-拮抗剂抑制。（4）肾上腺素在β-肾上腺素能阻断（普萘洛尔）后抑制脂解。（5）UK 14304的抗脂解作用受大鼠年龄的调节。

相似文献

1
Evidence of functional alpha 2-adrenergic receptors in adult-rat adipocytes by using the agonist UK 14304.使用激动剂UK 14304证明成年大鼠脂肪细胞中存在功能性α2-肾上腺素能受体。
Biochem J. 1988 Jun 15;252(3):679-82. doi: 10.1042/bj2520679.
2
Non-adrenergic sites for imidazolines are not directly involved in the alpha 2-adrenergic antilipolytic effect of UK 14304 in rat adipocytes.咪唑啉的非肾上腺素能位点不直接参与 UK 14304 在大鼠脂肪细胞中的α2 肾上腺素能抗脂解作用。
Biochem Pharmacol. 1990 Aug 1;40(3):437-45. doi: 10.1016/0006-2952(90)90541-r.
3
Increased alpha 2-adrenergic binding sites and antilipolytic effect in adipocytes from genetically obese rats.遗传性肥胖大鼠脂肪细胞中α2-肾上腺素能结合位点增加及抗脂解作用
J Lipid Res. 1990 May;31(5):811-9.
4
Characteristics of the alpha 2/beta-adrenoceptor-coupled adenylate cyclase system and their relationship with adrenergic responsiveness in hamster fat cells from different anatomical sites.来自不同解剖部位的仓鼠脂肪细胞中α2/β-肾上腺素能受体偶联腺苷酸环化酶系统的特征及其与肾上腺素能反应性的关系。
Eur J Biochem. 1992 Apr 15;205(2):867-73. doi: 10.1111/j.1432-1033.1992.tb16851.x.
5
Alpha 2-adrenergic binding and action in human adipocytes. Comparison between binding to plasma membrane preparations and to intact adipocytes.
Eur J Pharmacol. 1985 Dec 10;119(1-2):101-12. doi: 10.1016/0014-2999(85)90327-9.
6
Imidazolinic radioligands for the identification of hamster adipocyte alpha 2-adrenoceptors.用于鉴定仓鼠脂肪细胞α2-肾上腺素能受体的咪唑啉类放射性配体。
Eur J Pharmacol. 1989 Nov 21;171(2-3):145-57. doi: 10.1016/0014-2999(89)90103-9.
7
[Adrenergic lipolysis in human fat cells: properties and physiological role of alpha-adrenergic receptors (author's transl)].人脂肪细胞中的肾上腺素能脂肪分解：α-肾上腺素能受体的特性及生理作用（作者译）
J Physiol (Paris). 1980;76(2):133-46.
8
[Prolonged fasting on the lipolytic activity of isolated adipocytes of the rat epididymis].[长期禁食对大鼠附睾分离脂肪细胞脂解活性的影响]
C R Seances Soc Biol Fil. 1989;183(3):232-9.
9
Influence of androgenic status on the alpha 2/beta-adrenergic control of lipolysis in white fat cells: predominant alpha 2-antilipolytic response in testosterone-treated-castrated hamsters.雄激素状态对白色脂肪细胞脂解的α2/β - 肾上腺素能控制的影响：睾酮处理的去势仓鼠中主要的α2抗脂解反应
Endocrinology. 1988 Jun;122(6):2590-6. doi: 10.1210/endo-122-6-2590.
10
Late expression of alpha 2-adrenergic-mediated antilipolysis during differentiation of hamster preadipocytes.仓鼠前脂肪细胞分化过程中α2-肾上腺素能介导的抗脂解作用的晚期表达
J Lipid Res. 1991 Sep;32(9):1489-99.

引用本文的文献

1
Effects of the amino acid derivatives, β-hydroxy-β-methylbutyrate, taurine, and N-methyltyramine, on triacylglycerol breakdown in fat cells.氨基酸衍生物 β-羟基-β-甲基丁酸、牛磺酸和 N-甲基酪胺对脂肪细胞中三酰甘油分解的影响。
J Physiol Biochem. 2019 Aug;75(3):263-273. doi: 10.1007/s13105-019-00677-5. Epub 2019 Mar 27.
2
Interactions between an alpha2-adrenergic antagonist and a beta3-adrenergic agonist on the expression of UCP2 and UCP3 in rats.α2-肾上腺素能拮抗剂与β3-肾上腺素能激动剂对大鼠UCP2和UCP3表达的相互作用
J Physiol Biochem. 2002 Mar;58(1):17-23. doi: 10.1007/BF03179834.

本文引用的文献

1
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
J Biol Chem. 1964 Feb;239:375-80.
2
Alpha-2 adrenergic antilipolytic effect in dog fat cells: incidence of obesity and adipose tissue localization.α-2肾上腺素能对犬脂肪细胞的抗脂解作用：肥胖发生率与脂肪组织定位
Horm Metab Res. 1982 May;14(5):257-60. doi: 10.1055/s-2007-1018986.
3
Alpha-adrenergic responses in rabbit white fat cells: the influence of obesity and food restriction.
J Lipid Res. 1981 Sep;22(7):1084-93.
4
Ontogenic development of alpha-adrenergic receptors and responsiveness in white adipocytes.白色脂肪细胞中α-肾上腺素能受体的个体发生发育及反应性
Biochem J. 1980 Dec 15;192(3):947-50. doi: 10.1042/bj1920947.
5
Use of a novel rapid preparation of fat-cell plasma membranes employing Percoll to investigate the effects of insulin and adrenaline on membrane protein phosphorylation within intact fat-cells.利用采用Percoll的新型脂肪细胞质膜快速制备方法来研究胰岛素和肾上腺素对完整脂肪细胞内膜蛋白磷酸化的影响。
Biochem J. 1980 Nov 15;192(2):457-67. doi: 10.1042/bj1920457.
6
Pharmacological characterizations of adrenergic receptors in human adipocytes.人脂肪细胞中肾上腺素能受体的药理学特性
J Clin Invest. 1981 Feb;67(2):467-75. doi: 10.1172/JCI110055.
7
RX781094 a potent and selective alpha 2-adrenergic antagonist. Effects in adipocytes and hepatocytes.
Eur J Pharmacol. 1984 Apr 6;99(4):337-9. doi: 10.1016/0014-2999(84)90142-0.
8
Lack of functional antilipolytic alpha 2-adrenoceptor in rat fat cell:comparison with hamster adipocyte.大鼠脂肪细胞中缺乏功能性抗脂解α2-肾上腺素能受体：与仓鼠脂肪细胞的比较。
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;74(1):41-5. doi: 10.1016/0742-8413(83)90145-7.
9
UK-14,304, a potent and selective alpha2-agonist for the characterisation of alpha-adrenoceptor subtypes.UK-14,304，一种用于鉴定α-肾上腺素能受体亚型的强效选择性α2-激动剂。
Eur J Pharmacol. 1981 Jul 10;72(4):413-5. doi: 10.1016/0014-2999(81)90588-4.
10
Role of alpha 1 adrenoceptors in the turnover of phosphatidylinositol and of alpha 2 adrenoceptors in the regulation of cyclic AMP accumulation in hamster adipocytes.α1肾上腺素能受体在仓鼠脂肪细胞磷脂酰肌醇周转中的作用以及α2肾上腺素能受体在调节环磷酸腺苷积累中的作用。
Life Sci. 1980 Sep 15;27(11):953-61. doi: 10.1016/0024-3205(80)90105-8.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验