曲拉通WR-1339在植物溶酶体的细胞化学和生物化学特性研究中的应用。
Use of triton WR-1339 in cytochemical and biochemical characterization of phytolysosomes.
作者信息
Coulomb C, Coulom P J, Michel G
出版信息
Histochem J. 1982 Mar;14(2):175-96. doi: 10.1007/BF01041213.
PMID:6284682
Abstract
A new cytochemical assay of acid phosphatase activity employing Triton WR-1339, a non-ionic detergent, has been used to demonstrate the probable origin of the phytolysosome system and of the organization of the Golgi complex in the root meristem, Cucurbita pepo. The results were controlled biochemically by differential and linear density gradient ultracentrifugation of extracts from normal and Triton-treated roots. The various fractions were characterized by electron microscopy or by assay of marker enzymes.
摘要
一种采用非离子去污剂吐温WR - 1339的酸性磷酸酶活性新细胞化学测定法，已被用于证明西葫芦根分生组织中植物溶酶体系统的可能起源以及高尔基体复合体的组织情况。通过对正常根和经吐温处理的根提取物进行差速和线性密度梯度超速离心，对结果进行了生化对照。通过电子显微镜或标记酶测定对各个组分进行了表征。
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本文引用的文献
1
STUDIES ON THE INTRACELLULAR DIGESTIVE PROCESS IN MAMMALIAN TISSUE CULTURE CELLS.哺乳动物组织培养细胞的细胞内消化过程研究。
J Cell Biol. 1965 May 1;25(2):41-55. doi: 10.1083/jcb.25.2.41.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
GLUTARALDEHYDE STABILIZATION AS AN AID TO GOLGI APPARATUS ISOLATION.戊二醛固定作为高尔基体分离的辅助手段
Exp Cell Res. 1965 Jun;38:672-5. doi: 10.1016/0014-4827(65)90392-7.
4
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
5
Functions of lysosomes.溶酶体的功能。
Annu Rev Physiol. 1966;28:435-92. doi: 10.1146/annurev.ph.28.030166.002251.
6
Changes in lipid composition of triton-filled lysosomes during lysis. Association with activation of acid-active lipases and phospholipases.裂解过程中充满曲拉通的溶酶体脂质组成的变化。与酸性活性脂肪酶和磷脂酶的激活相关。
Biochim Biophys Acta. 1974 Jan 23;337(1):145-52. doi: 10.1016/0005-2760(74)90049-6.
7
Biochemical analysis of the pinocytotic process. 3. Subcellular distribution and metabolic effects of ( 3 H)Triton WR-1339.胞饮过程的生化分析。3. （3H）曲拉通WR-1339的亚细胞分布及代谢效应
Hoppe Seylers Z Physiol Chem. 1971 Oct;352(10):1347-58. doi: 10.1515/bchm2.1971.352.2.1347.
8
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9
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Exp Cell Res. 1975 Sep;94(2):363-76. doi: 10.1016/0014-4827(75)90503-0.
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