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Isolation of the kanamycin resistance region (Tn2350) of plasmid R1drd-19 as an autonomous replicon.

作者信息

Clerget M, Chandler M, Caro L

出版信息

J Bacteriol. 1982 Aug;151(2):924-31. doi: 10.1128/jb.151.2.924-931.1982.

DOI:10.1128/jb.151.2.924-931.1982
PMID:6284717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC220344/
Abstract

We have isolated a circular form of Tn2350, an IS1-flanked kanamycin resistance transposon forming part of the plasmid R1drd-19. This circle (pTn2350::9.6 kilobases) contains a single IS1 element and probably arises by recombination between the two directly repeated Is1 sequences of Tn2350. It can be used to transform Escherichia coli to kanamycin resistance. It is capable of autonomous replication but is not maintained stably in dividing cells and segregates under nonselective conditions. Cloning of a segment of pTn2350 on a conditional plasmid vector allowed us to assign the replication functions of this plasmid to a 1.6-kilobase restriction fragment. The plasmid R1drd-19 can thus be considered as a cointegrate between two replicons separated by IS1 sequences.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/220344/33eac41b08d7/jbacter00255-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/220344/c0a6b917dd7e/jbacter00255-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/220344/33eac41b08d7/jbacter00255-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/220344/c0a6b917dd7e/jbacter00255-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f9/220344/33eac41b08d7/jbacter00255-0409-a.jpg

相似文献

1
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引用本文的文献

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Mol Gen Genet. 1993 Sep;240(3):307-14. doi: 10.1007/BF00280380.
2
IS1-dependent generation of high-copy-number replicons from bacteriophage P1 Ap Cm as a mechanism of gene amplification.
J Bacteriol. 1986 May;166(2):609-17. doi: 10.1128/jb.166.2.609-617.1986.
3
Electron microscopic analysis of two nonconjugative derivatives of plasmid R1drd-19Km- from Escherichia coli.对来自大肠杆菌的质粒R1drd - 19Km - 的两种非接合衍生物进行电子显微镜分析。

本文引用的文献

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Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
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A novel role for site-specific recombination in maintenance of bacterial replicons.位点特异性重组在细菌复制子维持中的新作用。
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Production of extrachromosomal r-determinant circles from integrated R100.1: involvement of the E. coli recombination system.从整合的R100.1产生染色体外r-决定簇环：大肠杆菌重组系统的参与
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Mechanism of insertion and cointegration mediated by IS1 and Tn3.由IS1和Tn3介导的插入和共整合机制。
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:283-95. doi: 10.1101/sqb.1981.045.01.041.
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The structure of R1drd19: a revised physical map of the plasmid.R1drd19的结构：该质粒的修订物理图谱。
Mol Gen Genet. 1981;181(2):183-91. doi: 10.1007/BF00268425.
8
Isolation of an IS1 flanked kanamycin resistance transposon from R1drd19.从R1drd19中分离出一个侧翼为IS1的卡那霉素抗性转座子。
Mol Gen Genet. 1980;180(1):123-7. doi: 10.1007/BF00267360.
9
Plasmid replication functions: two distinct segments of plasmid R1, RepA and RepD, express incompatibility and are capable of autonomous replication.质粒复制功能：质粒R1的两个不同区段，即RepA和RepD，表现出不相容性且能够自主复制。
J Bacteriol. 1980 Dec;144(3):1126-38. doi: 10.1128/jb.144.3.1126-1138.1980.
10
Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance.细菌质粒在细胞分裂过程中的分配：一个实现稳定质粒遗传的顺式作用位点。
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