1型脊髓灰质炎病毒温度敏感突变体的分离与初步鉴定
Isolation and preliminary characterization of temperature-sensitive mutants of poliovirus type 1.
作者信息
Hewlett M J, Axelrod J H, Antinoro N, Feld R
出版信息
J Virol. 1982 Mar;41(3):1089-94. doi: 10.1128/JVI.41.3.1089-1094.1982.
Abstract
We isolated six temperature-sensitive mutants of poliovirus type 1 (Mahoney) by hydroxylamine mutagenesis and replica plating at 31, 33 (permissive), and 39 degrees C (restrictive). One of these mutants, designated tsB9, was chosen for more detailed examination. tsB9 accumulated 25% of the wild-type amount of virus-specific RNA at the restrictive temperature. We found that tsB9 was not able to synthesize mature, 35S single-stranded RNA at the restrictive temperature. In spite of the absence of significant RNA synthesis, tsB9 retained the ability to inhibit host protein synthesis during infection at 39 degrees C at about the same rate as wild-type virus.
摘要
我们通过羟胺诱变和在31、33℃（允许温度）和39℃（限制温度）下的影印培养，分离出了6株1型脊髓灰质炎病毒（Mahoney株）的温度敏感突变体。其中一个突变体，命名为tsB9，被选作更详细的研究对象。tsB9在限制温度下积累的病毒特异性RNA量为野生型的25%。我们发现tsB9在限制温度下无法合成成熟的35S单链RNA。尽管缺乏显著的RNA合成，但tsB9在39℃感染期间仍保留了抑制宿主蛋白合成的能力，其抑制速率与野生型病毒大致相同。
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