[位于β亚基结构基因之前的RNA聚合酶操纵子启动子的克隆]
[Cloning of the promoter of the RNA polymerase operon located before the structural gene of the beta-subunit].
作者信息
Bass I A, Kurenova E V, Mekhedov S L
出版信息
Mol Biol (Mosk). 1982 May-Jun;16(3):575-80.
PMID:6285178
Abstract
An EcoRI-fragment of the rpoBC operon carrying the end of the rplL gene, the intercistron region and the beginning of the rpoB gene cloned on the pBRH4 plasmid makes it tetracyclin-resistant, i. e. possesses the properties of a promoter. There is no appreciable difference in the degree of antibiotic resistance of recombinant plasmids carrying the main PJ promoter and the additional promoter.
摘要
携带rplL基因末端、基因间区域和rpoB基因起始部分的rpoBC操纵子的EcoRI片段克隆到pBRH4质粒上后，使其具有四环素抗性，即具有启动子的特性。携带主要PJ启动子和附加启动子的重组质粒在抗生素抗性程度上没有明显差异。
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