Hall L, Craig R K, Edbrooke M R, Campbell P N
Nucleic Acids Res. 1982 Jun 11;10(11):3503-15. doi: 10.1093/nar/10.11.3503.
Nucleotide sequence analyses of essentially full-length copies of human and guinea-pig pre-alpha-lactalbumin cDNAs contained within recombinant plasmids, (i) confirm the presence of 19 amino acid hydrophobic amino terminal peptide extensions encoded within each mRNA; and (ii) provides evidence for the existence of a minor variant of guinea-pig alpha-lactalbumin mRNA encoding a protein with a 36 residue carboxyl-terminal extension. Comparison of the nucleotide sequence within the coding region of the human, and the predominant guinea-pig pre-alpha-lactalbumin mRNAs, with the analogous region of hen pre-lysozyme mRNA provides compelling evidence that all have evolved from a common ancestral gene.
对重组质粒中包含的人及豚鼠前α-乳白蛋白cDNA的基本全长拷贝进行核苷酸序列分析，(i)证实每个mRNA中编码有19个氨基酸的疏水氨基末端肽延伸；(ii)为豚鼠α-乳白蛋白mRNA的一种小变体的存在提供了证据，该变体编码一种具有36个残基羧基末端延伸的蛋白质。将人及主要的豚鼠前α-乳白蛋白mRNA编码区的核苷酸序列与母鸡前溶菌酶mRNA的类似区域进行比较，提供了令人信服的证据，表明它们都从一个共同的祖先基因进化而来。
