Matsumoto K, Uno I, Oshima Y, Ishikawa T
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2355-9. doi: 10.1073/pnas.79.7.2355.
Mutants of Saccharomyces cerevisiae that require cAMP for growth have been isolated. Some of the mutants isolated were deficient in adenylate cyclase activity and mapped at a locus, cyr1, located near the centromere of chromosome X. Growth of cells carrying the cyr1 mutation was arrested at the G1 phase of the yeast cell cycle in the absence of cAMP. The cyr1 mutation was suppressed by a secondary mutation designated bcy1. The bcy1 mutation bypassed the need for cAMP for growth. The bcy1 mutants had extremely low levels of cAMP-binding protein and cAMP-dependent protein kinase but produced a high level of cAMP-independent protein kinase. The results indicate that cAMP is an essential factor for yeast cells to proceed through the cell cycle via the activation of protein kinase.
已分离出酿酒酵母中生长需要环磷酸腺苷（cAMP）的突变体。所分离出的一些突变体腺苷酸环化酶活性不足，定位于位于X染色体着丝粒附近的一个位点cyr1。在没有cAMP的情况下，携带cyr1突变的细胞生长在酵母细胞周期的G1期停滞。cyr1突变被一个名为bcy1的二次突变所抑制。bcy1突变绕过了生长对cAMP的需求。bcy1突变体的cAMP结合蛋白和cAMP依赖性蛋白激酶水平极低，但产生高水平的cAMP非依赖性蛋白激酶。结果表明，cAMP是酵母细胞通过激活蛋白激酶进行细胞周期所必需的因素。
