Matsumoto K, Uno I, Ishikawa T, Oshima Y
J Bacteriol. 1983 Nov;156(2):898-900. doi: 10.1128/jb.156.2.898-900.1983.
Yeast cells with a nonsense adenylate cyclase mutation, cyr1-3, required cyclic AMP for growth. This phenotype was suppressed by the byc1 mutation; however, cyr1-3 bcy1 cells produced no detectable level of adenylate cyclase or cyclic AMP. On induction, the bcy1 and cyr1-3 bcy1 mutant cells produced the same levels of galactokinase and alpha-D-glucosidase as did the wild-type cells and fourfold-higher levels of invertase. Since galactokinase synthesis was severely repressed by glucose in the constitutive GAL81 mutants, irrespective of the cyr1-3 bcy1 genotype, cyclic AMP may not be involved in catabolite repression.
带有无义腺苷酸环化酶突变（cyr1-3）的酵母细胞生长需要环磷酸腺苷（cAMP）。这种表型被byc1突变所抑制；然而，cyr1-3 bcy1细胞未检测到可检测水平的腺苷酸环化酶或环磷酸腺苷。诱导后，bcy1和cyr1-3 bcy1突变细胞产生的半乳糖激酶和α-D-葡萄糖苷酶水平与野生型细胞相同，蔗糖酶水平则高出四倍。由于在组成型GAL81突变体中，无论cyr1-3 bcy1基因型如何，半乳糖激酶的合成都会被葡萄糖严重抑制，因此环磷酸腺苷可能不参与分解代谢物阻遏。
