Ilonen J, Salmi A
Scand J Immunol. 1981 May;15(5):521-4. doi: 10.1111/j.1365-3083.1982.tb00679.x.
The amount of T-cell growth factor (TCGF) produced in vitro in antigen-stimulated lymphocyte cultures was measured by induction of growth in cell lines dependent on the factor. The method distinguished eight herpes-simplex-seronegative subjects from ten seropositive ones, and these results correlated with those of the lymphocyte blast transformation (LBT) test. The LBT responses and TCGF amounts produced in cultures with purified tuberculin (PPD) were also in good correlation (r = 0.833, P less than 0.001). Considerable levels of the factor were already present 12 h after the beginning of the culture. The method offers rapid quantification of cell-mediated immunity to specific antigens.
通过依赖该因子的细胞系生长诱导来测量抗原刺激的淋巴细胞培养物中体外产生的T细胞生长因子（TCGF）量。该方法区分了8名单纯疱疹血清阴性受试者和10名血清阳性受试者，这些结果与淋巴细胞增殖转化（LBT）试验的结果相关。用纯化结核菌素（PPD）培养产生的LBT反应和TCGF量也具有良好的相关性（r = 0.833，P < 0.001）。培养开始12小时后该因子就已达到相当水平。该方法可快速定量针对特定抗原的细胞介导免疫。
