Karttunen R, Andersson G, Ekre H P, Juutinen K, Surcel H M, Syrjälä H, Herva E
J Clin Microbiol. 1987 Jun;25(6):1074-8. doi: 10.1128/jcm.25.6.1074-1078.1987.
The T-cell response induced by Francisella tularensis antigen in sensitized subjects was characterized in vitro by measuring DNA synthesis in whole-blood and mononuclear cell cultures, interleukin 2 (IL-2) and gamma interferon (IFN-gamma) production, and IL-2 receptor expression. Correlations between these variables were estimated. The strengths of the responses were compared in 21 subjects naturally infected 2 years ago, 6 subjects vaccinated 5 to 6 years ago, and 13 control subjects with no history of infection or vaccination. Subjects with a history of natural infection synthesized more DNA in both whole-blood and mononuclear cell cultures, secreted more IL-2 and IFN-gamma, and expressed more IL-2 receptors than control subjects did. All these responses differed highly significantly (P less than 0.001) from those of the control subjects. The vaccinees exhibited somewhat lower responses than the naturally immunized subjects did, but the vaccinees could be distinguished from the control subjects by their DNA synthesis, receptor expression, and IFN-gamma production (P less than 0.01 to 0.001). The vaccinees showed a lower response, in terms of DNA synthesis and IL-2 secretion (P less than 0.05), than the infected group did but responded in a manner similar to that of this group, with respect to receptor positivity and IFN-gamma secretion (P greater than 0.10). The correlations between all the T-cell functions were good, with highly significant correlations (P less than 0.001) between whole-blood DNA synthesis and IL-2 and IFN-gamma secretion and between the two lymphokines (P less than 0.001). The results not only increase our knowledge of the T-cell response to tularemia antigen but also give an alternative approach to DNA synthesis measurement for the quantitation of T-cell responses. The results for the low-responding sensitized subjects seem to indicate that the parameters were comparable in sensitivity.
通过检测全血和单核细胞培养物中的DNA合成、白细胞介素2（IL-2）和γ干扰素（IFN-γ）的产生以及IL-2受体的表达，在体外对土拉弗朗西斯菌抗原诱导的致敏受试者的T细胞反应进行了表征。估计了这些变量之间的相关性。比较了21名2年前自然感染的受试者、6名5至6年前接种疫苗的受试者和13名无感染或疫苗接种史的对照受试者的反应强度。有自然感染史的受试者在全血和单核细胞培养物中合成的DNA更多，分泌的IL-2和IFN-γ更多，并且比对照受试者表达更多的IL-2受体。所有这些反应与对照受试者的反应均存在极显著差异（P小于0.001）。与自然免疫的受试者相比，接种疫苗者的反应略低，但接种疫苗者可通过其DNA合成、受体表达和IFN-γ产生与对照受试者区分开来（P小于0.01至0.001）。就DNA合成和IL-2分泌而言，接种疫苗者的反应低于感染组（P小于0.05），但就受体阳性和IFN-γ分泌而言，其反应方式与该组相似（P大于0.10）。所有T细胞功能之间的相关性良好，全血DNA合成与IL-2和IFN-γ分泌之间以及两种细胞因子之间存在极显著相关性（P小于0.001）。这些结果不仅增加了我们对T细胞对土拉菌病抗原反应的了解，还为T细胞反应定量提供了一种测量DNA合成的替代方法。低反应致敏受试者的结果似乎表明这些参数在敏感性方面具有可比性。
