Yonemasu K, Shinkai H, Sasaki T
Coll Relat Res. 1981 Jul;1(4):385-90. doi: 10.1016/s0174-173x(81)80015-5.
The hydroxylysine-glycosides in bovine and mouse C1q are directly quantified in parallel with those in human C1q after the alkaline hydrolysis of these molecules. Human, bovine and mouse C1q contain 68.3, 66.3 and 64.0 hydroxylysine-galactosylglucose residues in each of these molecules respectively. Only human C1q contains 2.5 residues of hydroxylysine-galactose per molecule, and both of bovine and mouse C1q contain no detectable hydroxylysine-monosaccharides in their molecules. The percentage of hydroxylysine residues glycosylated to total hydroxylysine residues in each of these molecules is calculated to be 86.4, 92.0 and 95.1% for human, bovine and mouse C1q respectively and is comparable with each other. The percentages of hydroxylysine residues resistant to periodate oxidation to total hydroxylysine residues in these molecules were 61.1, 65.3 and 74.3% for human, bovine and mouse C1q respectively and were significantly lower than those estimated by the direct quantification of hydroxylysine-glycosides after the alkaline hydrolysis of these molecules.
在对牛和小鼠C1q分子进行碱性水解后，将它们的羟赖氨酸糖苷与人类C1q的羟赖氨酸糖苷同时进行直接定量分析。人类、牛和小鼠的C1q分子中，每个分子分别含有68.3、66.3和64.0个羟赖氨酸-半乳糖基葡萄糖残基。只有人类C1q分子每个含有2.5个羟赖氨酸-半乳糖残基，而牛和小鼠的C1q分子中均未检测到羟赖氨酸单糖。计算得出，这些分子中糖基化的羟赖氨酸残基占总羟赖氨酸残基的百分比，人类、牛和小鼠的C1q分别为86.4%、92.0%和95.1%，三者相互可比。这些分子中对高碘酸盐氧化有抗性的羟赖氨酸残基占总羟赖氨酸残基的百分比，人类、牛和小鼠的C1q分别为61.1%、65.3%和74.3%，显著低于对这些分子进行碱性水解后通过直接定量羟赖氨酸糖苷所估算的百分比。
