牛角膜基质胶原蛋白一些溴化氰裂解片段的分离及羟赖氨酸糖苷含量
Isolation and hydroxylysine glycoside content of some cyanogen bromide-cleaved fragments of collagen from bovine corneal stroma.
作者信息
Panjwani N A, Harding J J
出版信息
Biochem J. 1978 Jun 1;171(3):687-695. doi: 10.1042/bj1710687.
Abstract
Six CNBr-cleaved fragments of insoluble collagen from bovine cornea were isolated, characterized and examined for hydroxylysine glycosides. Thus the general distribution of most of the glycoside along the collagen molecule was determined. Collagen from bovine stroma is almost entirely type I. This work forms a basis for the pinpointing of glycoside-attachment sites along the collagen molecules of bovine cornea.
摘要
从牛角膜中分离出六个经溴化氰裂解的不溶性胶原蛋白片段，对其进行表征并检测羟赖氨酸糖苷。由此确定了大部分糖苷沿胶原蛋白分子的总体分布。牛基质中的胶原蛋白几乎全是I型。这项工作为确定沿牛角膜胶原蛋白分子的糖苷附着位点奠定了基础。
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