Eggena P, Reinach P
Endocrinology. 1982 Sep;111(3):1001-9. doi: 10.1210/endo-111-3-1001.
The natriferic and hydroosmotic responses to vasopressin (ADH) of the isolated toad bladder were studied as a function of the serosal bath NaCl concentration. Bladders were preincubated in Ringer's solution containing equiosmotic substitutions of mannitol, choline chloride, or choline sulfate for NaCl. Their hydroosmotic and natriferic responses to ADH were measured in NaCl Ringer's solution. Bladders previously bathed on their serosal surface with mannitol had a markedly diminished hydroosmotic response, whereas the natriferic response of these bladders was essentially normal. Similarly, preincubation with either choline chloride or sulfate also inhibited the hydroosmotic response, although the decline was substantially less than that with mannitol. Preincubation with mannitol also inhibited the hydroosmotic response to dibutyryl cAMP. Therefore, the isosmotic substitution of serosal bath NaCl with mannitol decreases primarily the hydroosmotic response to ADH at a post-AMP step. The natriferic effect of the hormone remains essentially intact under these conditions. Different compartments (or cell types) appear to regulate the action of ADH on sodium and on water transport in this tissue because of the selective effects of preincubation with low NaCl Ringer's solution on the hydroosmotic and natriferic responses to ADH.
研究了离体蟾蜍膀胱对血管加压素（抗利尿激素，ADH）的钠分泌和水渗透反应与浆膜浴NaCl浓度的关系。将膀胱预先在含有等渗替代NaCl的甘露醇、氯化胆碱或硫酸胆碱的林格氏溶液中孵育。在NaCl林格氏溶液中测量它们对ADH的水渗透和钠分泌反应。先前在其浆膜表面用甘露醇处理过的膀胱，其水渗透反应明显减弱，而这些膀胱的钠分泌反应基本正常。同样，用氯化胆碱或硫酸胆碱预孵育也会抑制水渗透反应，尽管下降幅度远小于甘露醇处理的情况。用甘露醇预孵育也会抑制对二丁酰cAMP的水渗透反应。因此，用甘露醇等渗替代浆膜浴NaCl主要在AMP后步骤降低对ADH的水渗透反应。在这些条件下，该激素的钠分泌作用基本保持完整。由于用低NaCl林格氏溶液预孵育对ADH的水渗透和钠分泌反应有选择性影响，不同的区室（或细胞类型）似乎调节ADH对该组织中钠和水转运的作用。
