人乳腺癌中25(OH)D3和1,25(OH)2D3的特异性胞质结合蛋白。
Specific cytosol binding proteins for 25(OH)D3 and 1.25(OH)2D3 in human breast cancers.
作者信息
Nakata M, Tsuboi S, Takenaka M, Konishi Y, Kawashima R, Tomita M, Nakagawa T, Iwamura N, Fujita T
出版信息
Endocrinol Jpn. 1981 Dec;28(6):741-6. doi: 10.1507/endocrj1954.28.741.
Binding proteins for 1.25 (OH) 2D3 were investigated in thirty breast cancers. Human breast cancer was shown to contain specific, high affinity cytosol binding proteins for 1.25 (OH) 2D3 and 25 (OH) D3. The binding protein for 1.25 (OH) 2D3 sedimented at 3.7 S and the binding protein for 25 (OH) D3 at about 6.0 S on sucrose density gradient analysis containing 0.3 M KCl and 1 mM dithiothreitol in buffer. Kd for 1.25 (OH) 2D3 were from 0.1 x 10(-11) M to 7.1 x 10(-11) M measured by Scatchard plots. Competition binding studies indicated that the relative specificity of the binding protein for 1.25 (OH) 2D3 much greater than 25 (OH) D3 greater than 1 alpha (OH) D3, 24,25 (OH)2D3 greater than D3 much greater than Estradiol-17 beta. 1.25 (OH) 2D3 receptor-positive was detected in twenty-eight out of thirty breast cancers.
在三十例乳腺癌中研究了1,25-二羟维生素D3（1,25(OH)2D3）的结合蛋白。研究表明，人乳腺癌组织中含有对1,25(OH)2D3和25-羟维生素D3（25(OH)D3）具有特异性、高亲和力的胞质结合蛋白。在含有0.3M氯化钾和1mM二硫苏糖醇的缓冲液中进行蔗糖密度梯度分析时，1,25(OH)2D3的结合蛋白在3.7S处沉降，25(OH)D3的结合蛋白在约6.0S处沉降。通过Scatchard图测定，1,25(OH)2D3的解离常数（Kd）为0.1×10⁻¹¹M至7.1×10⁻¹¹M。竞争结合研究表明，结合蛋白对1,25(OH)2D3的相对特异性远大于25(OH)D3大于1α-羟维生素D3、24,25-二羟维生素D3大于维生素D3远大于雌二醇-17β。三十例乳腺癌中有二十八例检测到1,25(OH)2D3受体阳性。
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