Enzymatic syntheses of deoxyribo- and ribonucleoside triphosphates were observed with purified, chromatin bound enzyme C from Morris Hepatoma 9121 nuclei. 2. Intermediate substrates were nucleoside mono- and diphosphates for enzyme C. 3. The three phosphate residues of ATP serving as energy-rich phosphate donor were successively transferred to a nucleoside substrate by enzyme C.