用物理化学和生化技术对莫里斯肝癌9121细胞染色质中的六种核苷 - 核苷酸磷酸转移酶进行表征。

Characterization of six nucleoside-nucleotide phosphotransferases from the chromatin of Morris hepatoma 9121 cells by physicochemical and biochemical techniques.

Lenger K

Int J Biochem. 1982;14(10):955-60. doi: 10.1016/0020-711x(82)90081-7.

Abstract

Six nucleoside-nucleotide phosphotransferases A-F were isolated from chromatin of Morris Hepatoma 9121 cells and characterized. 2. The apparent mol wt are: 13,000 daltons for A, 35,000 or 70,000 for B and E, 240,000 for C, 180,000 for D and 300,000-400,000 for F. 3. The sedimentation values are: 2.5-3 S for A, 3 or 5 S for B and E, 11 S for C, 7-8 S for D and 14 S for F. 4. The activity of the six enzymes A-F was stimulated with 1-2.5 micrograms calf thymus DNA using thymidine/ATP as substrates. 5. In the presence of defined polydeoxynucleotides, each enzyme A-F showed a differential preference for its substrates.

摘要

从莫里斯肝癌9121细胞的染色质中分离并鉴定出六种核苷 - 核苷酸磷酸转移酶A - F。2. 其表观分子量分别为：A为13,000道尔顿，B和E为35,000或70,000，C为240,000，D为180,000，F为300,000 - 400,000。3. 沉降值分别为：A为2.5 - 3 S，B和E为3或5 S，C为11 S，D为7 - 8 S，F为14 S。4. 以胸苷/ATP为底物，用1 - 2.5微克小牛胸腺DNA刺激六种酶A - F的活性。5. 在特定的多脱氧核苷酸存在下，每种酶A - F对其底物表现出不同的偏好。