Bvui：一种来自普通芽孢杆菌的位点特异性核酸内切酶。

Bvui: a site-specific endonuclease from Bacillus vulgatis.

作者信息

Beaty J S, McLean-Howen C A, Brown L R

出版信息

Gene. 1982 Apr;18(1):61-7. doi: 10.1016/0378-1119(82)90056-7.

Abstract

A site-specific endodeoxyribonuclease was partially purified from an extract of osmotically shocked spheroplasts of a Bacillus vulgatis strain; the enzyme has been designated BvuI and its activity was characterized. The locations of BvuI-generated cleavages on bacteriophage lambda and M13 derivatives mp7, mp8 and mp9, SV40 and PBR322 DNA molecules were determined. BvuI was shown to recognize the DNA sequence decreases 5'-G-Pu-G-C-Py-C-3' 3'-C-Py-C-G-Pu-G-5' increases and cleaves it at the positions indicated by arrows. Two identical BvuI recognition sites separated by fourteen nucleotide pairs were shown to occur within the tetracycline resistance gene of PBR322; BvuI should be useful for molecular cloning in that plasmid vector.

摘要

从一株普通芽孢杆菌经渗透压休克处理的原生质体提取物中部分纯化出一种位点特异性脱氧核糖核酸内切酶；该酶被命名为BvuI，并对其活性进行了表征。确定了BvuI在噬菌体λ、M13衍生物mp7、mp8和mp9、SV40以及PBR322 DNA分子上产生切割的位置。结果表明，BvuI识别的DNA序列为5'-G-Pu-G-C-Py-C-3' 3'-C-Py-C-G-Pu-G-5'，并在箭头所示位置进行切割。结果显示，在PBR322的四环素抗性基因内存在两个由14个核苷酸对隔开的相同BvuI识别位点；BvuI在该质粒载体的分子克隆中应具有实用价值。