Treisman R, Cowie A, Favaloro J, Jat P, Kamen R
J Mol Appl Genet. 1981;1(2):83-92.
The polyoma virus early region mRNAs synthesized during productive infection of mouse cells have been characterized at the nucleotide level. One- and two-dimensional agarose gel fractionation of nuclease S1-resistant RNA-DNA hybrids was used to establish basic structures. The two splice donors and the two splice acceptors were positioned more precisely by high resolution S1-gel mapping with terminally labeled DNA probes and polyacrylamide gels. The nucleotide sequences across the three splice joints were established by cloning and sequencing partial cDNA copies of the mRNAs. In combination with data on the polyadenylated 3'-end previously published, and the detailed analysis of the capped 5'-ends presented elsewhere, the present data complete the description of a family of differentially spliced mRNAs able to encode the known early region gene products, small-T, middle-T, and large-T proteins.
在小鼠细胞生产性感染期间合成的多瘤病毒早期区域mRNA已在核苷酸水平上进行了表征。使用核酸酶S1抗性RNA-DNA杂交体的一维和二维琼脂糖凝胶分级分离来确定基本结构。通过使用末端标记的DNA探针和聚丙烯酰胺凝胶进行高分辨率S1凝胶图谱分析，更精确地定位了两个剪接供体和两个剪接受体。通过克隆和测序mRNA的部分cDNA拷贝，确定了跨越三个剪接接头的核苷酸序列。结合先前发表的关于聚腺苷酸化3'末端的数据，以及在其他地方对加帽5'末端的详细分析，目前的数据完成了对一组能够编码已知早期区域基因产物、小T、中T和大T蛋白的差异剪接mRNA的描述。
