Ross L J
J Gen Virol. 1982 Jun;60(Pt 2):375-80. doi: 10.1099/0022-1317-60-2-375.
A Marek's disease lymphoblastoid cell line (MSB-1) has been analysed by immunoprecipitation for expression of tumour-associated antigen, Marek's disease virus (MDV)-specific antigens and antigens specific to avian leukosis-sarcoma viruses. Rabbit antisera raised against two independently derived cell lines after extensive absorption with normal chick cells reacted with a polypeptide of mol. wt. 40 000 (40K) in extracts of MSB-1 cells. The 40K polypeptide was not present in myeloblasts or in chick embryo fibroblasts (CEF) infected with MDV and did not react with antiserum raised against normal chicken thymus antigens. The possibility that the 40K polypeptide is a tumour-associated antigen is discussed. Seven MDV-specific antigens were noted in infected CEF (mol. wt. 110K, 100K, 80K, 70K, 50K, 35K and 32K) but none of these was detected in MSB-1 cells. The avian leukosis-sarcoma group-specific antigen P27gag and its precursor Pr76gag were not found in MSB-1 cells, confirming that expression of mature gag protein is not required for transformation by MDV. However, two polypeptides of unknown origin and function (mol. wt. 180K and 110K) were precipitated from MSB-1 cells with a rabbit anti-Rous sarcoma (Schmidt-Rupin, subgroup D) antiserum.
利用免疫沉淀法对一种马立克氏病淋巴母细胞系（MSB - 1）进行了分析，以检测肿瘤相关抗原、马立克氏病病毒（MDV）特异性抗原以及禽白血病 - 肉瘤病毒特异性抗原的表达情况。用正常鸡细胞大量吸收后针对两个独立衍生细胞系制备的兔抗血清，与MSB - 1细胞提取物中分子量为40000（40K）的一种多肽发生反应。40K多肽不存在于成髓细胞或感染MDV的鸡胚成纤维细胞（CEF）中，且不与针对正常鸡胸腺抗原制备的抗血清发生反应。文中讨论了40K多肽是肿瘤相关抗原的可能性。在感染的CEF中发现了七种MDV特异性抗原（分子量分别为110K、100K、80K、70K、50K、35K和32K），但在MSB - 1细胞中均未检测到。MSB - 1细胞中未发现禽白血病 - 肉瘤组特异性抗原P27gag及其前体Pr76gag，这证实了MDV转化并不需要成熟gag蛋白的表达。然而，用兔抗劳斯肉瘤病毒（施密特 - 鲁平，D亚群）抗血清从MSB - 1细胞中沉淀出了两种来源和功能未知的多肽（分子量分别为180K和110K）。
