Sharma J M, Witter R L, Coulson B D
J Natl Cancer Inst. 1978 Nov;61(5):1273-80. doi: 10.1093/jnci/61.5.1273.
Chickens inoculated with herpesvirus of turkeys or with apathogenic or attenuated vaccine strains of Marek's disease virus (MDV) developed a T-cell-mediated immune response to Marek's disease (MD) tumor cells. This immune response was detected in a 4-hour 51Cr-release assay in which effector cells obtained from spleens of vaccinated chickens were reacted with 51Cr-labeled target cells of an MD lymphoblastoid cell line (MSB-1). The cytotoxic effector cells generated by the vaccine viruses had characteristics similar to those noted previously for anti-MSB-1 effector cells generated by MDV. The immune response was specific to MSB-1 cells, because another target cell line (TLT) antigenically unrelated to MSB-1 cells was not lysed by the effector cells nor did the unrelated target cells inhibit the cytotoxicity of effector cells against MSB-1 target in a cold-target inhibition assay. Because MSB-1 cells contain MD tumor-associated surface antigen, we postulated that the immune response detected in the vaccinated chickens may be directed against this antigen and that the antitumor antigen immunity may play a role in the mechanism of vaccine protection against lymphoma development by pathogenic MDV.
接种火鸡疱疹病毒或马立克氏病病毒（MDV）的无致病性或减毒疫苗株的鸡，对马立克氏病（MD）肿瘤细胞产生了T细胞介导的免疫反应。这种免疫反应在一项4小时的51Cr释放试验中被检测到，在该试验中，从接种疫苗的鸡脾脏中获得的效应细胞与51Cr标记的MD淋巴母细胞系（MSB-1）的靶细胞反应。疫苗病毒产生的细胞毒性效应细胞具有与先前MDV产生的抗MSB-1效应细胞相似的特征。这种免疫反应对MSB-1细胞具有特异性，因为另一种与MSB-1细胞抗原无关的靶细胞系（TLT）既没有被效应细胞裂解，在冷靶抑制试验中，无关靶细胞也没有抑制效应细胞对MSB-1靶细胞的细胞毒性。由于MSB-1细胞含有MD肿瘤相关表面抗原，我们推测在接种疫苗的鸡中检测到的免疫反应可能针对该抗原，并且抗肿瘤抗原免疫可能在疫苗预防致病性MDV引起的淋巴瘤发生的机制中发挥作用。
