McComb R D, Jones T R, Pizzo S V, Bigner D D
J Neuropathol Exp Neurol. 1982 Sep;41(5):479-89.
The sarcomatous components of most glioma-sarcomas are thought to arise from the neoplastic transformation of hyperplastic endothelial and adventitial vascular cells in a preexisting glioblastoma multiforme. The expression of factor VIII/von Willebrand factor (FVIII/vWF), a marker for endothelial cells, and of glial fibrillary acidic protein (GFAP), a marker for glial cells, was examined in 10 glioblastomas and seven mixed glioma-sarcomas using the peroxidase-antiperoxidase immunohistochemical technique. Hyperplasia of small blood vessels was observed in all 10 glioblastomas; in five, the vascular proliferation had resulted in the formation of prominent glomeruloid structures. FVIII/vWF was detected in the endothelial cells in these vascular structures, but not in the adventitial cells. In the mixed glioma-sarcomas. FVIII/vWF was detected only in endothelial cells; there was no staining for FVIII/vWF in the neoplastic mesenchymal cells. The gliomatous components of the mixed tumors stained intensely for GFAP. These observations indicate that both endothelial and nonendothelial cell types contribute to the small vessel hyperplasia in glioblastomas, and that the sarcomatous components of mixed glioma-sarcomas are derived from either non-endothelial cells or endothelial cells that have undergone antigenic loss following transformation.
大多数胶质瘤-肉瘤的肉瘤成分被认为源自预先存在的多形性胶质母细胞瘤中增生的内皮细胞和外膜血管细胞的肿瘤性转化。使用过氧化物酶-抗过氧化物酶免疫组织化学技术，对10例胶质母细胞瘤和7例混合性胶质瘤-肉瘤检测了内皮细胞标志物因子VIII/血管性血友病因子（FVIII/vWF）以及胶质细胞标志物胶质纤维酸性蛋白（GFAP）的表达情况。在所有10例胶质母细胞瘤中均观察到小血管增生；其中5例中，血管增生导致形成明显的肾小球样结构。在这些血管结构的内皮细胞中检测到FVIII/vWF，但在外膜细胞中未检测到。在混合性胶质瘤-肉瘤中，FVIII/vWF仅在内皮细胞中检测到；肿瘤性间充质细胞中未检测到FVIII/vWF染色。混合性肿瘤的胶质成分GFAP染色强烈。这些观察结果表明，内皮细胞和非内皮细胞类型均参与了胶质母细胞瘤中的小血管增生，并且混合性胶质瘤-肉瘤的肉瘤成分源自非内皮细胞或转化后发生抗原丢失的内皮细胞。
