Kochi N, Budka H
Acta Neuropathol. 1987;73(2):124-30. doi: 10.1007/BF00693777.
The expression of glial fibrillary acidic protein, fibronectin (FN), factor VIII-related antigen (FVIII/RAG), and of three monohistiocytic markers, lysozyme, alpha-1-antitrypsin and alpha-1-antichymotrypsin was examined in five gliosarcomas (GS) by peroxidase-antiperoxidase immunostaining of formalin-fixed and paraffin-embedded specimens, and compared with vascular changes in 16 glioblastomas (GB). In contrast to GB, endothelial proliferations of GS were sheathed by sarcomatous tissue (perivascular sarcoma), which was contiguous with fibrosarcomatous areas. Cells with conspicuous intracytoplasmic FN content (FN+ cells) were seen in the vascular stroma of GB and dominated in the sarcomatous parts of GS. Most FN+ cells of GS were of varying size and shape and clearly neoplastic. Monohistiocytic markers were demonstrable in small infiltrating mononuclear cells as well as in many sarcomatous cells including FN+ cells. FVIII/RAG was restricted to lumen-lining endothelium and was not found in sarcomatous cells. These results suggest that a major part of sarcoma in GS is less likely to develop from proliferated endothelial cells than from histiocytic cells in the perivascular spaces of GB. By FN mediation, histiocytic cells might also guide and promote sarcomatous proliferations of other mesenchymal cells, leading to fibrosarcomatous development. Prominent monstrous giant cells of one GS seemed to be degenerating glioma cells.
通过对福尔马林固定、石蜡包埋标本进行过氧化物酶-抗过氧化物酶免疫染色，检测了5例胶质肉瘤（GS）中胶质纤维酸性蛋白、纤连蛋白（FN）、因子VIII相关抗原（FVIII/RAG）以及三种单核巨噬细胞标志物（溶菌酶、α1-抗胰蛋白酶和α1-抗糜蛋白酶）的表达，并与16例胶质母细胞瘤（GB）的血管变化进行了比较。与GB不同，GS的内皮细胞增殖被肉瘤组织（血管周围肉瘤）包绕，该肉瘤组织与纤维肉瘤区域相连。在GB的血管基质中可见胞质内FN含量明显的细胞（FN+细胞），且在GS的肉瘤部分占主导地位。GS的大多数FN+细胞大小和形状各异，明显为肿瘤性细胞。单核巨噬细胞标志物在小的浸润性单核细胞以及许多肉瘤细胞（包括FN+细胞）中均可显示。FVIII/RAG仅限于血管内衬内皮细胞，在肉瘤细胞中未发现。这些结果表明，GS中肉瘤的主要部分不太可能由增殖的内皮细胞发展而来，而更可能由GB血管周围间隙中的组织细胞发展而来。通过FN介导，组织细胞也可能引导和促进其他间充质细胞的肉瘤增殖，导致纤维肉瘤的发展。1例GS中突出的怪异巨细胞似乎是退变的胶质瘤细胞。
