两栖动物角膜氯离子转运的药理学分析。
A pharmacological analysis of chloride transport across the amphibian cornea.
作者信息
Bentley P J, McGahan M C
出版信息
J Physiol. 1982 Apr;325:481-92. doi: 10.1113/jphysiol.1982.sp014163.
Abstract
- Active Cl, but not Na, transport across the toad cornea was inhibited by mepacrine, which is a phospholipase A2 inhibitor; trifluoperazine, which blocks the action of calmodulin; and meclofenamic acid, which inhibits synthesis of prostaglandins. Bumetanide and DIDS (4,4'-diisothiocyano-2,2'-stilbene disulphonic acid) have previously been shown to inhibit this Cl transport. The interactions of these antagonists with several agonists that increase Cl transport were studied. 2. The effects of adrenaline, prostaglandin E2, dibutyryl cyclic AMP (DBcAMP) and the Ca ionophore A23187 were inhibited by mepacrine, trifluoperazine and bumetanide. 3. The inhibitory effects of high concentrations of DIDS, however, could be overcome by all of the agonists except DBcAMP. 4. Meclofenamic acid only blocked the effects of A23187. 5. A model is proposed to account for the observed actions and interactions of the various antagonists and agonists on Cl transport. This involves possible roles for Ca, calmodulin and phospholipase A2.
摘要
- 氯的主动转运（而非钠的主动转运）跨越蟾蜍角膜时会受到以下物质的抑制：米帕林，它是一种磷脂酶A2抑制剂；三氟拉嗪，它能阻断钙调蛋白的作用；以及甲氯芬那酸，它能抑制前列腺素的合成。先前已表明布美他尼和4,4'-二异硫氰酸-2,2'-二苯乙烯二磺酸（DIDS）可抑制这种氯转运。研究了这些拮抗剂与几种能增加氯转运的激动剂之间的相互作用。2. 肾上腺素、前列腺素E2、二丁酰环磷酸腺苷（DBcAMP）和钙离子载体A23187的作用均受到米帕林、三氟拉嗪和布美他尼的抑制。3. 然而，除DBcAMP外，所有激动剂均可克服高浓度DIDS的抑制作用。4. 甲氯芬那酸仅阻断A23187的作用。5. 提出了一个模型来解释所观察到的各种拮抗剂和激动剂对氯转运的作用及相互作用。这涉及钙、钙调蛋白和磷脂酶A2可能发挥的作用。
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本文引用的文献
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