二异丙基氨基磺酸钠（DIDS）对两栖动物角膜氯离子转运的抑制作用。
Inhibitory action of DIDS on chloride transport across the amphibian cornea.
作者信息
Bentley P J, McGahan M C
出版信息
J Physiol. 1980 Jul;304:519-27. doi: 10.1113/jphysiol.1980.sp013340.
Abstract
- DIDS (4,4'-diisothiocyano-2,2'-stilbene disulphonic acid) reduced the CI Isc across the toad's (Bufo marinus) cornea. It acted on either the aqueous or tear side and these effects were additive. 2. The reduction in the Isc was equivalent to the decline in the undirectional flux of Cl from the aqueous to the tear side. The Cl flux from tear to aqueous was not changed. 3. DIDS did not change transmural Na transport. 4. The action of the diuretic bumetanide, which also inhibits Cl transport was additive to that of DIDS when both compounds were present on the aqueous though not when they were on the tear side of the cornea. 5. The results are consistent with the role of a Cl-/anion exchange mechanism in active Cl transport across the cornea. 6. A hypothesis regarding the interactions and site of action of bumetanide in relation to that of DIDS, and the Cl transport process, is proposed.
摘要
- 4,4'-二异硫氰基-2,2'-二苯乙烯二磺酸（DIDS）降低了蟾蜍（海蟾蜍）角膜的氯离子跨上皮电流（CI Isc）。它作用于房水侧或泪液侧，且这些效应是相加的。2. Isc的降低等同于氯离子从房水侧向泪液侧单向通量的下降。从泪液到房水的氯离子通量未改变。3. DIDS不改变跨膜钠转运。4. 利尿剂布美他尼也抑制氯离子转运，当两种化合物都存在于房水侧时，其作用与DIDS相加，但当它们存在于角膜泪液侧时则不然。5. 结果与氯离子/阴离子交换机制在角膜主动氯离子转运中的作用一致。6. 提出了一个关于布美他尼与DIDS相互作用及作用位点以及氯离子转运过程的假说。
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