Isle H D, Emerson S U
J Virol. 1982 Jul;43(1):37-40. doi: 10.1128/JVI.43.1.37-40.1982.
A hybrid infectivity assay specific for primary transcription was developed to analyze the production of functional mRNAs by vesicular stomatitis virus. A template prepared from wild-type virions of the New Jersey serotype of vesicular stomatitis virus was reconstituted with RNA polymerase proteins from the wild type or temperature-sensitive mutants, and the in vivo temperature sensitivity of the polymerase was determined by infectivity assay. The data demonstrate that the New Jersey temperature-sensitive mutants A1 and E1 have non-temperature-sensitive transcriptases, whereas the B1 and F1 mutants both have temperature-sensitive L proteins which are defective in primary transcription.
开发了一种针对初级转录的杂交感染性测定法，以分析水疱性口炎病毒功能性mRNA的产生。用来自野生型或温度敏感突变体的RNA聚合酶蛋白重建了由水疱性口炎病毒新泽西血清型的野生型病毒粒子制备的模板，并通过感染性测定法确定了聚合酶在体内的温度敏感性。数据表明，新泽西温度敏感突变体A1和E1具有非温度敏感的转录酶，而B1和F1突变体都具有在初级转录中存在缺陷的温度敏感L蛋白。
