使用混合感染性测定法分析水疱性口炎病毒新泽西血清型温度敏感突变体的初级转录。
Use of a hybrid infectivity assay to analyze primary transcription of temperature-sensitive mutants of the New Jersey serotype of vesicular stomatitis virus.
作者信息
Isle H D, Emerson S U
出版信息
J Virol. 1982 Jul;43(1):37-40. doi: 10.1128/JVI.43.1.37-40.1982.
Abstract
A hybrid infectivity assay specific for primary transcription was developed to analyze the production of functional mRNAs by vesicular stomatitis virus. A template prepared from wild-type virions of the New Jersey serotype of vesicular stomatitis virus was reconstituted with RNA polymerase proteins from the wild type or temperature-sensitive mutants, and the in vivo temperature sensitivity of the polymerase was determined by infectivity assay. The data demonstrate that the New Jersey temperature-sensitive mutants A1 and E1 have non-temperature-sensitive transcriptases, whereas the B1 and F1 mutants both have temperature-sensitive L proteins which are defective in primary transcription.
摘要
开发了一种针对初级转录的杂交感染性测定法，以分析水疱性口炎病毒功能性mRNA的产生。用来自野生型或温度敏感突变体的RNA聚合酶蛋白重建了由水疱性口炎病毒新泽西血清型的野生型病毒粒子制备的模板，并通过感染性测定法确定了聚合酶在体内的温度敏感性。数据表明，新泽西温度敏感突变体A1和E1具有非温度敏感的转录酶，而B1和F1突变体都具有在初级转录中存在缺陷的温度敏感L蛋白。
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Use of a hybrid infectivity assay to analyze primary transcription of temperature-sensitive mutants of the New Jersey serotype of vesicular stomatitis virus.使用混合感染性测定法分析水疱性口炎病毒新泽西血清型温度敏感突变体的初级转录。
J Virol. 1982 Jul;43(1):37-40. doi: 10.1128/JVI.43.1.37-40.1982.
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本文引用的文献
1
Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype.水泡性口炎病毒新泽西血清型温度敏感突变体的分离与鉴定
J Virol. 1971 Dec;8(6):836-41. doi: 10.1128/JVI.8.6.836-841.1971.
2
Proposed replicative role of the NS polypeptide of vesicular stomatitis virus: structural analysis of an electrophoretic variant.水疱性口炎病毒NS多肽的拟复制作用：一种电泳变体的结构分析
J Virol. 1979 Jul;31(1):8-15. doi: 10.1128/JVI.31.1.8-15.1979.
3
Temperature-sensitive mutants of complementation group E of vesicular stomatitis virus New Jersey serotype possess altered NS polypeptides.水疱性口炎病毒新泽西血清型E互补组的温度敏感突变体具有改变的NS多肽。
J Virol. 1979 Aug;31(2):325-33. doi: 10.1128/JVI.31.2.325-333.1979.
4
Effect of temperature-sensitive mutation on activity of the RNA transcriptase of vesicular stomatitis virus New Jersey.温度敏感突变对水疱性口炎病毒新泽西株RNA转录酶活性的影响。
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