Pringle C R, Duncan I B, Stevenson M
J Virol. 1971 Dec;8(6):836-41. doi: 10.1128/JVI.8.6.836-841.1971.
Forty-eight temperature-sensitive (ts) mutants have been isolated from a wild-type strain of the New Jersey serotype of vesicular stomatitis virus (VSV) after exposure to the base analogue mutagen 5-fluorouracil. Of these mutants, 47 have been classified into 6 nonoverlapping complementation groups containing 21, 17, 4, 3, 2, and 1 mutant, respectively (1 mutant remaining unallocated). The ribonucleic acid (RNA) phenotype of 23 of these mutants has been established. Four of the six groups contain one or more mutants unable to synthesize detectable amounts of viral RNA under restrictive conditions (39 C). No complementation was observed in mixed infection with ts mutants from the five established complementation groups of the Indiana serotype of VSV.
在用碱基类似物诱变剂5-氟尿嘧啶处理水泡性口炎病毒（VSV）新泽西血清型的野生型毒株后，分离出了48个温度敏感（ts）突变体。在这些突变体中，47个已被分为6个不重叠的互补群，分别包含21、17、4、3、2和1个突变体（1个突变体未分配）。已确定其中23个突变体的核糖核酸（RNA）表型。六个互补群中的四个包含一个或多个在限制条件（39℃）下无法合成可检测量病毒RNA的突变体。在用VSV印第安纳血清型五个已确定的互补群中的ts突变体进行混合感染时，未观察到互补现象。
