Hoggan M D, Buckler C E, Sears J F, Chan H W, Rowe W P, Martin M A
J Virol. 1982 Jul;43(1):8-17. doi: 10.1128/JVI.43.1.8-17.1982.
The internal organization of endogenous xenotropic murine leukemia virus proviruses was determined in a series of blot hybridization experiments in which DNA from several different inbred mouse strains, digested with restriction enzymes known to cleave xenotropic proviral DNAs at least twice, was annealed to generalized murine leukemia virus or xenotropic env-specific DNA probes. Comigrating bands of variable intensity which hybridized to the xenotropic env probe were identified in all inbred mouse DNA preparations. At least seven classes of endogenous xenotropic proviral DNA with respect to SacI cleavage maps were detected in mouse DNA. Two of the seven classes were indistinguishable from proviruses associated with known infectious xenotropic murine leukemia viruses. These results are consistent with the existence of related but organizationally distinct families of endogenous xenotropic proviral DNA that are present in different relative abundances in mouse genomic DNA.
在一系列印迹杂交实验中确定了内源性嗜异性鼠白血病病毒前病毒的内部组织，在这些实验中，来自几种不同近交系小鼠品系的DNA用已知至少能切割嗜异性前病毒DNA两次的限制性酶消化后，与通用鼠白血病病毒或嗜异性env特异性DNA探针退火。在所有近交系小鼠DNA制剂中都鉴定出了与嗜异性env探针杂交的迁移率相同但强度可变的条带。在小鼠DNA中检测到至少七类关于SacI切割图谱的内源性嗜异性前病毒DNA。这七类中的两类与与已知感染性嗜异性鼠白血病病毒相关的前病毒无法区分。这些结果与内源性嗜异性前病毒DNA相关但组织上不同的家族的存在一致，这些家族以不同的相对丰度存在于小鼠基因组DNA中。
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